Marked increase in urinary excretion of apolipoproteins in children with nephrolithiasis associated with hypercalciuria

Larisa Kovacevic; Hong Lu; Joseph A Caruso; Tuhina Govil-Dalela; Ronald Thomas; Yegappan Lakshmanan

doi:10.1007/s00467-016-3576-1

Back

Marked increase in urinary excretion of apolipoproteins in children with nephrolithiasis associated with hypercalciuria

Journal article

Peer reviewed

Marked increase in urinary excretion of apolipoproteins in children with nephrolithiasis associated with hypercalciuria

Larisa Kovacevic, Hong Lu, Joseph A Caruso, Tuhina Govil-Dalela, Ronald Thomas and Yegappan Lakshmanan

Pediatric nephrology (Berlin, West), Vol.32(6), pp.1029-1033

06/2017

DOI: 10.1007/s00467-016-3576-1

PMID: 28188435

View Online

Abstract

Using a proteomic approach, we aimed to identify and compare the urinary excretion of proteins involved in lipid transport and metabolism in children with kidney stones and hypercalciuria (CAL), hypocitraturia (CIT), and normal metabolic work-up (NM), and in healthy controls (HCs). Additionally, we aimed to confirm these results using ELISA, and to examine the relationship between the urinary excretion of selected proteins with demographic, dietary, blood, and urinary parameters. Prospective, controlled, pilot study of pooled urine from CAL, CIT, and NM versus age- and gender-matched HCs, using liquid chromatography-mass spectrometry. Relative protein abundance was estimated using spectral counting. Results were confirmed by ELISA performed on individual samples. Of the 1,813 proteins identified, 230 met the above criteria. Of those, 5 proteins (apolipoprotein A-II [APOA2]; apolipoprotein A-IV [APOA4]; apolipoprotein C-III [APOA3]; fatty acid-binding protein, liver [FABPL]; fatty acid-binding protein, adipocyte [FABP4]) involved in lipid metabolism and transport were found in the CAL group, with significant differences compared with HCs. ELISA analysis indicated statistically significant differences in the urinary excretion of APOC3, APOA4, and FABPL in the CAL group compared with HCs. Twenty-four-hour urinary calcium excretion correlated significantly with concentrations of ApoC3 (r = 0.77, p < 0.001), and FABPL (r = 0.80, p = 0.005). We provide proteomic data showing increased urinary excretion of lipid metabolism/transport-related proteins in children with kidney stones and hypercalciuria. These findings suggest that abnormalities in lipid metabolism might play a role in kidney stone formation.

Adolescent

Apolipoproteins - metabolism

Apolipoproteins - urine

Calcium - metabolism

Calcium - urine

Child

Chromatography, Liquid

Citrates - urine

Enzyme-Linked Immunosorbent Assay

Fatty Acid-Binding Proteins - metabolism

Female

Humans

Hypercalciuria - metabolism

Hypercalciuria - urine

Kidney Calculi - metabolism

Kidney Calculi - urine

Male

Mass Spectrometry

Pilot Projects

Prospective Studies

Proteomics - methods

Renal Elimination

Details

Title: Subtitle: Marked increase in urinary excretion of apolipoproteins in children with nephrolithiasis associated with hypercalciuria
Creators: Larisa Kovacevic - Children's Hospital of Michigan
Hong Lu - Children's Hospital of Michigan
Joseph A Caruso - Wayne State University
Tuhina Govil-Dalela - Children's Hospital of Michigan
Ronald Thomas - Children's Hospital of Michigan
Yegappan Lakshmanan - Children's Hospital of Michigan
Resource Type: Journal article
Publication Details: Pediatric nephrology (Berlin, West), Vol.32(6), pp.1029-1033
DOI: 10.1007/s00467-016-3576-1
PMID: 28188435
NLM abbreviation: Pediatr Nephrol
ISSN: 0931-041X
eISSN: 1432-198X
Grant note: P30 CA022453 / NCI NIH HHS P30 ES020957 / NIEHS NIH HHS S10 OD010700 / NIH HHS
Language: English
Date published: 06/2017
Academic Unit: Stead Family Department of Pediatrics
Record Identifier: 9984961027002771

Metrics

2 Record Views

See more details