Journal article
Mast Cell Deficiency Limits the Development of Chronic Rhinosinusitis in Mice
Annals of otology, rhinology & laryngology, Vol.125(4), pp.290-296
04/2016
DOI: 10.1177/0003489415610775
PMID: 26681624
Abstract
Chronic rhinosinusitis (CRS) is one of the most common chronic diseases in adults in both developing and developed countries. The etiology and pathogenesis of CRS remain poorly understood, and the disease is refractory to therapy in many patients. Mast cell activation has been demonstrated in the sinonasal mucosa of patients with CRS; however, the specific contribution of mast cells to the development and pathogenesis of this disease has not been established.
The objective of this study was to investigate the role of mast cells in the development of CRS.
C57BL/6 wild-type and C57BL/6-Kit(W-sh/W-sh) mast cell-deficient mice were immunized by intraperitoneal allergen injection and subsequent chronic low dose intranasal allergen challenges. The sinonasal phenotypes of these groups were then evaluated and compared to saline-treated controls using radiologic, histologic, and immunologic methods.
Wild-type mice exposed to chronic intranasal allergen developed many features seen in human CRS, including mucosal thickening, cystic changes, polyp development, eosinophilia, goblet cell hyperplasia, and mast cell activation. In contrast, sinonasal pathology was significantly attenuated in mast cell-deficient mice subjected to the same chronic allergen protocol. Specifically, tissue eosinophilia and goblet cell hyperplasia were reduced by approximately 50% compared to wild-type levels. Surprisingly, none of the mast cell-deficient mice subjected to chronic allergen challenge developed cystic changes or polypoid changes in the nose or sinuses.
These data identify a critical role for mast cells in the development of many features of a mouse model of eosinophilic CRS, suggesting that therapeutic strategies targeting mast cells be examined in humans afflicted with this disease.
Details
- Title: Subtitle
- Mast Cell Deficiency Limits the Development of Chronic Rhinosinusitis in Mice
- Creators
- Xiaoyang Hua - University of IowaWarren C Naselsky - University of North Carolina at Chapel HillCorey M Jania - University of North Carolina at Chapel HillKelly D Chason - University of North Carolina at Chapel HillJulianne J Huang - University of North Carolina at Chapel HillClaire M Doerschuk - University of North Carolina at Chapel HillScott M Graham - University of IowaBrent A Senior - University of North Carolina at Chapel HillStephen L Tilley - University of North Carolina at Chapel Hill
- Resource Type
- Journal article
- Publication Details
- Annals of otology, rhinology & laryngology, Vol.125(4), pp.290-296
- DOI
- 10.1177/0003489415610775
- PMID
- 26681624
- ISSN
- 0003-4894
- eISSN
- 1943-572X
- Grant note
- 1R21AI096139-01A1 / NIAID NIH HHS U19AI077437 / NIAID NIH HHS 2T32DC000040-21 / NIDCD NIH HHS HL071802 / NHLBI NIH HHS
- Language
- English
- Date published
- 04/2016
- Academic Unit
- Neurosurgery; Otolaryngology
- Record Identifier
- 9984303897702771
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