Journal article
Mast cell–induced immunopathology in recurrent pregnancy losses
American journal of reproductive immunology (1989), Vol.82(1), pp.e13128-n/a
07/2019
DOI: 10.1111/aji.13128
PMID: 31006153
Abstract
Problem: Mast cells (MC) have been known to play an important role in inflammation and angiogenesis by secreting numerous mediators, such as proteases, gelatinases, and proteoglycans. Three different MC subtypes were found in the endometrial layers of the uterus. In this study, we aim to investigate the role of endometrial MCs in recurrent pregnancy losses (RPL).
Method of study: Endometrial biopsy was performed 5-7 days post-ovulation (implantation window) in women with a history of two or more RPL (n = 46) and normal fertile women (n = 10). Quantitative RT-PCR was performed to detect the expression of various mast cell mediators. Endometrial samples were evaluated using immunohistochemistry for c-kit receptor (CD117) and tryptase (MC activation marker).
Results: Mast cells were present throughout the entire layers of the endometrium; their count was elevated in RPL patients as compared to controls. The gene expression of c-Kit receptor was not different between the study groups. There are significant increases in the mRNA expression of various mediators, that is, stem cell factor (P = 0.029), tryptase (P = 0.024), heparan sulfate (P = 0.0005), and MMP-2 (P < 0.0001) in women with RPL as compared to normal controls. Chymase gene expression was not detected in most of the endometrial samples.
Conclusion: This study has shown that MCs are overactive in RPL patients by creating a pro-inflammatory milieu, suggesting a novel role in the immunopathology of RPL. Future studies are needed to better understand the role of MC in implantation and placental angiogenesis.
Keywords: MMP-2; endometrium; mast cell; recurrent pregnancy loss; tryptase.
Details
- Title: Subtitle
- Mast cell–induced immunopathology in recurrent pregnancy losses
- Creators
- Youssef Derbala - Rosalind Franklin University of Medicine and ScienceHaidy Elazzamy - Rosalind Franklin University of Medicine and ScienceMahmood Bilal - Rosalind Franklin University of Medicine and ScienceRachel Reed - Rosalind Franklin University of Medicine and ScienceMaria Dinorah Salazar Garcia - Rosalind Franklin University of Medicine and ScienceAnnie Skariah - Rosalind Franklin University of Medicine and ScienceSvetlana Dambaeva - Rosalind Franklin University of Medicine and ScienceEmilio Fernandez - Clínica Las CondesAlfredo Germain - Clínica Las CondesAlice Gilman‐Sachs - Rosalind Franklin University of Medicine and ScienceKenneth Beaman - Rosalind Franklin University of Medicine and ScienceJoanne Kwak‐Kim - Rosalind Franklin University of Medicine and Science
- Resource Type
- Journal article
- Publication Details
- American journal of reproductive immunology (1989), Vol.82(1), pp.e13128-n/a
- DOI
- 10.1111/aji.13128
- PMID
- 31006153
- ISSN
- 1046-7408
- eISSN
- 1600-0897
- Language
- English
- Date published
- 07/2019
- Academic Unit
- Pathology
- Record Identifier
- 9984183998302771
Metrics
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