Maternal Tgif1 regulates nodal gene expression in Xenopus

Tyler C Kerr; Tawny N Cuykendall; Laura C Luettjohann; Douglas W Houston

doi:10.1002/dvdy.21707

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Maternal Tgif1 regulates nodal gene expression in Xenopus

Journal article

Open access

Peer reviewed

Maternal Tgif1 regulates nodal gene expression in Xenopus

Tyler C Kerr, Tawny N Cuykendall, Laura C Luettjohann and Douglas W Houston

Developmental dynamics, Vol.237(10), pp.2862-2873

10/2008

DOI: 10.1002/dvdy.21707

PMID: 18816846

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Abstract

In Xenopus, the maternal transcription factor VegT is necessary and sufficient to initiate the expression of nodal-related genes, which are central to many aspects of early development. However, little is known about regulation of VegT activity. Using maternal loss-of-function experiments, we show that the maternal homeoprotein, Tgif1, antagonizes VegT and plays a central role in anteroposterior patterning by negatively regulating a subset of nodal-related genes. Depletion of Tgif1 causes the anteriorization of embryos and the up-regulation of nodal paralogues nr5 and nr6. Furthermore, Tgif1 inhibits activation of nr5 by VegT in a manner that requires a C-terminal Sin3 corepressor-interacting domain. Tgif1 has been implicated in the transcriptional corepression of transforming growth factor-beta (TGFbeta) and retinoid signaling. However, we show that Tgif1 does not inhibit these pathways in early development. These results identify an essential role for Tgif1 in the control of nodal expression and provide insight into Tgif1 function and mechanisms controlling VegT activity.

Retinoids - metabolism

Xenopus Proteins - genetics

Homeodomain Proteins - metabolism

Humans

Embryo, Nonmammalian - metabolism

Gene Expression Regulation, Developmental - genetics

Molecular Sequence Data

Embryo, Nonmammalian - embryology

Nodal Protein - metabolism

Mothers

Xenopus Proteins - chemistry

Nodal Protein - genetics

Gene Deletion

Smad2 Protein - genetics

Female

Xenopus laevis - genetics

Amino Acid Sequence

Xenopus laevis - embryology

Signal Transduction

Animals, Genetically Modified

RNA, Messenger - genetics

Smad2 Protein - metabolism

Homeodomain Proteins - chemistry

Homeodomain Proteins - genetics

Sequence Homology, Amino Acid

Sequence Alignment

Animals

Xenopus Proteins - deficiency

Xenopus laevis - metabolism

Xenopus Proteins - metabolism

Details

Title: Subtitle: Maternal Tgif1 regulates nodal gene expression in Xenopus
Creators: Tyler C Kerr - University of Iowa, Department of Biology, Iowa City, Iowa 52246-1324, USA
Tawny N Cuykendall
Laura C Luettjohann
Douglas W Houston
Resource Type: Journal article
Publication Details: Developmental dynamics, Vol.237(10), pp.2862-2873
Publisher: United States
DOI: 10.1002/dvdy.21707
PMID: 18816846
ISSN: 1058-8388
eISSN: 1097-0177
Language: English
Date published: 10/2008
Academic Unit: Biology
Record Identifier: 9983992087402771

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