Journal article
Mathematical model for the luminol chemiluminescence reaction catalyzed by peroxidase
Biotechnology and bioengineering, Vol.41(11), pp.1112-1120
05/1993
DOI: 10.1002/bit.260411115
PMID: 18601298
Abstract
A kinetic model that accurately describes intensity vs. time reaction profiles for the chemiluminescence reaction between luminol and hydrogen peroxide, as catalyzed by horseradish peroxidase, is derived and evaluated. A set of three differential equations is derived and solved to provide intensity time information for the first 200 seconds of the reaction. The model accurately predicts intensity-time profiles when literature values are used for all but one of the reaction rate constants. Furthermore, the model predicts a nonlinear curve for plots of light intensity versus the initial hydrogen peroxide concentration. Experimental data confirm that such plots are nonlinear. Finally, a linear double-reciprocal plot is predicted by the model and the experimental data verify this relationship.
Details
- Title: Subtitle
- Mathematical model for the luminol chemiluminescence reaction catalyzed by peroxidase
- Creators
- Lin Li - Department of Chemistry, University of lowa City, lowa 52242Mark A Arnold - Department of Chemistry, University of lowa City, lowa 52242Jonathan S Dordick - Department of Chemical and Biochemical Engineering, University of lowa City, lowa 52242
- Resource Type
- Journal article
- Publication Details
- Biotechnology and bioengineering, Vol.41(11), pp.1112-1120
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company
- DOI
- 10.1002/bit.260411115
- PMID
- 18601298
- ISSN
- 0006-3592
- eISSN
- 1097-0290
- Number of pages
- 9
- Language
- English
- Date published
- 05/1993
- Academic Unit
- Center for Biocatalysis and Bioprocessing; Fraternal Order of Eagles Diabetes Research Center; Chemistry
- Record Identifier
- 9984216712902771
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