Journal article
Matrix-Enhanced Calibration Procedure for Multivariate Calibration Models with Near-Infrared Spectra
Applied spectroscopy, Vol.52(10), pp.1339-1347
10/1998
DOI: 10.1366/0003702981942672
Abstract
A novel method is introduced for developing calibration models for the spectroscopic measurement of chemical concentrations in an aqueous environment. To demonstrate this matrix-enhanced calibration procedure, we developed calibration models to quantitate glucose and glutamine concentrations in an insect cell culture medium that is a complex mixture of more than 20 components, with three components that manifest significant concentration changes. Accurate calibration models were generated for glucose and glutamine by using a calibration data set composed of 60 samples containing the analytes dissolved in an aqueous buffer along with as few as two samples of the analytes dissolved in culture medium. Standard errors of prediction were 1.0 mM for glucose and 0.35 mM for glutamine. The matrix-enhanced method was also applied to culture medium samples collected during the course of a second bioreactor run. Addition of three culture medium samples to a buffer calibration reduced glucose prediction errors from 3.8 mM to 1.0 mM; addition of two culture medium samples reduced glutamine prediction errors from 1.6 mM to 0.76 mM. Results from this study suggest that spectroscopic calibration models can be developed from a relatively simple set of samples provided that some account for variations in the sample matrix.
Details
- Title: Subtitle
- Matrix-Enhanced Calibration Procedure for Multivariate Calibration Models with Near-Infrared Spectra
- Creators
- Mark R Riley - Department of Chemical and Biochemical Engineering (M.R.R., D.W.M.) and Department of Chemistry (M.A.A.), The University of Iowa, Iowa City, Iowa 52242Mark A Arnold - Department of Chemical and Biochemical Engineering (M.R.R., D.W.M.) and Department of Chemistry (M.A.A.), The University of Iowa, Iowa City, Iowa 52242David W Murhammer - Department of Chemical and Biochemical Engineering (M.R.R., D.W.M.) and Department of Chemistry (M.A.A.), The University of Iowa, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- Applied spectroscopy, Vol.52(10), pp.1339-1347
- DOI
- 10.1366/0003702981942672
- ISSN
- 0003-7028
- eISSN
- 1943-3530
- Publisher
- SAGE Publications; London, England
- Language
- English
- Date published
- 10/1998
- Academic Unit
- Center for Biocatalysis and Bioprocessing; Chemistry; Chemical and Biochemical Engineering
- Record Identifier
- 9984003929902771
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