Matrix Metalloproteinase Response of Dendritic Cell, Gingival Epithelial Keratinocyte, and T-Cell Transwell Co-Cultures Treated with Porphyromonas gingivalis Hemagglutinin-B

Amber M Bates; Carol L Fischer; Vrushali P Abhyankar; Georgia K Johnson; Janet M Guthmiller; Ann Progulske-Fox; Kim A Brogden

doi:10.3390/ijms19123923

Back

Matrix Metalloproteinase Response of Dendritic Cell, Gingival Epithelial Keratinocyte, and T-Cell Transwell Co-Cultures Treated with Porphyromonas gingivalis Hemagglutinin-B

Journal article

Open access

Peer reviewed

Matrix Metalloproteinase Response of Dendritic Cell, Gingival Epithelial Keratinocyte, and T-Cell Transwell Co-Cultures Treated with Porphyromonas gingivalis Hemagglutinin-B

Amber M Bates, Carol L Fischer, Vrushali P Abhyankar, Georgia K Johnson, Janet M Guthmiller, Ann Progulske-Fox and Kim A Brogden

International journal of molecular sciences, Vol.19(12), p.3923

12/07/2018

DOI: 10.3390/ijms19123923

PMCID: PMC6321455

PMID: 30544510

Files and links (1)

url

https://doi.org/10.3390/ijms19123923View

Published (Version of record) Open Access

Abstract

Matrix metalloproteinases (MMPs) are enzymes involved in periodontal tissue destruction. Hemagglutinin B (HagB) from the periodontal pathogen induces an elevated MMP response in dendritic cells, but responses from cultures of single-cell types do not reflect the local tissue environment. The objective of this study was to measure HagB-induced MMP responses in a transwell co-culture system containing dendritic cells, gingival epithelial (GE) keratinocytes, and CD4+ T-cells. Transwell co-cultures were assembled and treated with or without HagB. Immunoassays were used to determine production of MMP1, MMP7, MMP9, and MMP12 in response to HagB up to 64 h. Control responses were subtracted from HagB-induced responses. A two-way fixed effect ANOVA was fit to log-transformed concentrations and pairwise group comparisons were conducted ( < 0.05). At 64 h, dendritic cells produced elevated MMP1 and MMP9 responses, which were attenuated in the 3-cell co-culture ( < 0.05). There were also significant differences in MMP7 and MMP12 production between single-cell cultures and co-cultures. These results support the need to use multiple cell types in culture models to evaluate a more representative response to proinflammatory agonists. This three-cell transwell co-culture model may help us better understand the inflammatory process in periodontal disease and test novel therapeutic approaches.

Coculture Techniques

Humans

CD4-Positive T-Lymphocytes - metabolism

Keratinocytes - cytology

Porphyromonas gingivalis - chemistry

Keratinocytes - drug effects

T-Lymphocytes - cytology

T-Lymphocytes - metabolism

Keratinocytes - metabolism

T-Lymphocytes - drug effects

Gingiva - cytology

Dendritic Cells - drug effects

Hemagglutinins - pharmacology

Dendritic Cells - cytology

Cell Culture Techniques

Matrix Metalloproteinases - metabolism

Matrix Metalloproteinase 1 - metabolism

Dendritic Cells - metabolism

Details

Title: Subtitle: Matrix Metalloproteinase Response of Dendritic Cell, Gingival Epithelial Keratinocyte, and T-Cell Transwell Co-Cultures Treated with Porphyromonas gingivalis Hemagglutinin-B
Creators: Amber M Bates - Iowa Institute for Oral Health Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA. ambates@wisc.edu
Carol L Fischer - Department of Biology, Waldorf University, Forest City, IA 50436, USA. carol.fischer@waldorf.edu
Vrushali P Abhyankar - Department of Periodontology, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN 38103, USA. vabhyank@uthsc.edu
Georgia K Johnson - Department of Periodontics, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA. georgia-johnson@uiowa.edu
Janet M Guthmiller - College of Dentistry, University of Nebraska Medical Center, Lincoln, NE 68583, USA. janet.guthmiller@unmc.edu
Ann Progulske-Fox - Center for Molecular Microbiology and Department of Oral Biology, University of Florida, Gainesville, FL 32603, USA. APFOX@dental.ufl.edu
Kim A Brogden - Department of Periodontics, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA. kim-brogden@uiowa.edu
Resource Type: Journal article
Publication Details: International journal of molecular sciences, Vol.19(12), p.3923
DOI: 10.3390/ijms19123923
PMID: 30544510
PMCID: PMC6321455
NLM abbreviation: Int J Mol Sci
ISSN: 1661-6596
eISSN: 1422-0067
Publisher: Switzerland
Grant note: T90 DE023520 / NIDCR NIH HHS T90 DE023520 / National Institute of Dental and Craniofacial Research R01 DE014390 / National Institute of Dental and Craniofacial Research
Language: English
Date published: 12/07/2018
Academic Unit: Periodontics
Record Identifier: 9984065801802771

Metrics

30 Record Views

14 Times Cited - Web of Science