Maturation and localization of macrophages and microglia during infection with a neurotropic murine coronavirus

Steven P Templeton; Taeg S Kim; Katherine O'Malley; Stanley Perlman

doi:10.1111/j.1750-3639.2007.00098.x

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Maturation and localization of macrophages and microglia during infection with a neurotropic murine coronavirus

Journal article

Open access

Peer reviewed

Maturation and localization of macrophages and microglia during infection with a neurotropic murine coronavirus

Steven P Templeton, Taeg S Kim, Katherine O'Malley and Stanley Perlman

Brain pathology (Zurich, Switzerland), Vol.18(1), pp.40-51

01/2008

DOI: 10.1111/j.1750-3639.2007.00098.x

PMCID: PMC7596182

PMID: 17935605

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Abstract

Macrophages and microglia are critical in the acute inflammatory response and act as final effector cells of demyelination during chronic infection with the neutrotropic MHV-JHM strain of mouse hepatitis virus (MHV-JHM). Herein, we show that "immature" F4/80(+)Ly-6C(hi) monocytes are the first cells, along with neutrophils, to enter the MHV-JHM-infected central nervous system (CNS). As the infection progresses, macrophages in the CNS down-regulate expression of Ly-6C and CD62L, consistent with maturation, and a higher frequency express CD11c, a marker for dendritic cells (DCs). Microglia also express CD11c during this phase of the infection. CD11c(+) macrophages in the infected CNS exhibit variable properties of immature antigen-presenting cells (APCs), with modestly increased CD40 and MHC expression, and equivalent potent antigen uptake when compared with CD11c(-) macrophages. Furthermore, CDllc(+) and F4/80(+) macrophages and microglia are localized to areas of demyelination, in some instances directly associated with damaged axons. These results suggest that chronic CNS infection results in the appearance of CD11c-expressing macrophages from the blood that exhibit properties of immature APCs, are closely associated with areas of demyelination, and may act as final effectors of myelin destruction.

Humans

Brain - virology

CD11 Antigens - immunology

Macrophages - virology

Microglia - immunology

Demyelinating Diseases - immunology

Microglia - pathology

Demyelinating Diseases - pathology

Macrophages - immunology

Disease Models, Animal

Biomarkers - metabolism

Demyelinating Diseases - physiopathology

Macrophages - pathology

Myelin Sheath - immunology

Myelin Sheath - pathology

Encephalitis - virology

Mice, Inbred C57BL

Biomarkers - analysis

Encephalitis - pathology

Encephalitis - immunology

Mice, Transgenic

Murine hepatitis virus - pathogenicity

Coronavirus Infections - immunology

Cell Differentiation - immunology

Animals

Coronavirus Infections - virology

Axons - pathology

Microglia - virology

Coronavirus Infections - pathology

Brain - pathology

Mice

HeLa Cells

Axons - immunology

Chemotaxis, Leukocyte - immunology

Brain - immunology

Axons - virology

Details

Title: Subtitle: Maturation and localization of macrophages and microglia during infection with a neurotropic murine coronavirus
Creators: Steven P Templeton - University of Iowa, Iowa City, IA, USA
Taeg S Kim
Katherine O'Malley
Stanley Perlman
Resource Type: Journal article
Publication Details: Brain pathology (Zurich, Switzerland), Vol.18(1), pp.40-51
DOI: 10.1111/j.1750-3639.2007.00098.x
PMID: 17935605
PMCID: PMC7596182
NLM abbreviation: Brain Pathol
ISSN: 1015-6305
eISSN: 1750-3639
Publisher: Switzerland
Grant note: R01 NS40438 / NINDS NIH HHS
Language: English
Date published: 01/2008
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777475302771

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