Journal article
Maturation of the Oral Microbiome in Caries-Free Toddlers: A Longitudinal Study
Journal of dental research, Vol.99(2), pp.159-167
02/01/2020
DOI: 10.1177/0022034519889015
PMCID: PMC6977153
PMID: 31771395
Abstract
Understanding the development of the oral microbiota in healthy children is of great importance to oral and general health. However, limited data exist on a healthy maturation of the oral microbial ecosystem in children. Moreover, the data are biased by mislabeling "caries-free" populations. Therefore, we aimed to characterize the healthy salivary and dental plaque microbiome in young children. Caries-free (ICDAS [International Caries Detection and Assessment System] score 0) children (n = 119) and their primary caregivers were followed from 1 until 4 y of child age. Salivary and dental plaque samples were collected from the children at 3 time points (T1, 1 y old; T2, 2.5 y old; and T3, 4 y old). Only saliva samples were collected from the caregivers. Bacterial V4 16S ribosomal DNA amplicons were sequenced using Illumina MiSeq. The reads were denoised and mapped to the zero-radius operational taxonomic units (zOTUs). Taxonomy was assigned using HOMD. The microbial profiles of children showed significant differences (P = 0.0001) over time. Various taxa increased, including Fusobacterium, Actinomyces, and Corynebacterium, while others showed significant decreases (e.g., Alloprevotella and Capnocytophaga) in their relative abundances over time. Microbial diversity and child-caregiver similarity increased most between 1 and 2.5 y of age while still not reaching the complexity of the caregivers at 4 y of age. The microbiome at 1 y of age differed the most from those at later time points. A single zOTU (Streptococcus) was present in all samples (n = 925) of the study. A large variation in the proportion of shared zOTUs was observed within an individual child over time (2% to 42% of zOTUs in saliva; 2.5% to 38% in dental plaque). These findings indicate that the oral ecosystem of caries-free toddlers is highly heterogeneous and dynamic with substantial changes in microbial composition over time and only few taxa persisting across the 3 y of the study. The salivary microbiome of 4-y-old children is still distinct from that of their caregivers.
Details
- Title: Subtitle
- Maturation of the Oral Microbiome in Caries-Free Toddlers: A Longitudinal Study
- Creators
- D. Kahharova - Academic Center for Dentistry AmsterdamB. W. Brandt - Academic Center for Dentistry AmsterdamM. J. Buijs - Academic Center for Dentistry AmsterdamM. Peters - University of MichiganR. Jackson - Indiana University – Purdue University IndianapolisG. Eckert - Indiana University – Purdue University IndianapolisB. Katz - Indiana University – Purdue University IndianapolisM. A. Keels - Duke UniversityS. M. Levy - Univ Iowa, Iowa City, IA USAM. Fontana - University of MichiganE. Zaura - Academic Center for Dentistry Amsterdam
- Resource Type
- Journal article
- Publication Details
- Journal of dental research, Vol.99(2), pp.159-167
- DOI
- 10.1177/0022034519889015
- PMID
- 31771395
- PMCID
- PMC6977153
- NLM abbreviation
- J Dent Res
- ISSN
- 0022-0345
- eISSN
- 1544-0591
- Publisher
- Sage
- Number of pages
- 9
- Grant note
- 5U01DE021412 / National Health Institute (NIH); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Stichting Bevordering Tandheelkundige Kennis with NTvT Onderzoeksbeurs 2017 UL1-TR000442; 2UL1-TR000433; UL1-TR000006 / NIH CTSA grants Delta Dental of Wisconsin Renaissance Health Service Corporation for Delta Dental of Michigan Colgate University of Michigan, School of Dentistry; University of Michigan System Delta Dental of Iowa ACTA Research Institute
- Language
- English
- Date published
- 02/01/2020
- Academic Unit
- Preventive and Community Dentistry; Epidemiology
- Record Identifier
- 9984367734402771
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