Journal article
Measurement of Aryl and Alcohol Sulfotransferase Activity
Current Protocols in Toxicology, Vol.8(1), pp.4.5.1-4.5.9
05/2001
DOI: 10.1002/0471140856.tx0405s08
Abstract
Two methods are presented for determining the catalytic activity of aryl and alcohol sulfotransferases. The first assay is a simple and rapid procedure that is based on the extraction of a paired ion formed between the product organic sulfate and methylene blue. The second method employs HPLC analysis of the substrate‐dependent formation of PAP, an assay that is particularly useful when the sulfuric acid ester product is chemically unstable.
Details
- Title: Subtitle
- Measurement of Aryl and Alcohol Sulfotransferase Activity
- Creators
- Jonathan J. Sheng - University of IowaVyas Sharma - University of IowaMichael W. Duffel - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Current Protocols in Toxicology, Vol.8(1), pp.4.5.1-4.5.9
- DOI
- 10.1002/0471140856.tx0405s08
- ISSN
- 1934-9254
- eISSN
- 1934-9262
- Number of pages
- 9
- Language
- English
- Date published
- 05/2001
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984303287002771
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