Journal article
Measurement of Mn SOD and Cu Zn SOD Activity in Mammalian Tissue Homogenates
Current Protocols in Toxicology, Vol.8(1), pp.7.5.1-7.5.11
05/2001
DOI: 10.1002/0471140856.tx0705s08
Abstract
Three basic forms of mammalian SODs exist and they are distinguished by their sizes and locations. SOD enzyme activity is not easily monitored by direct measurement because the substrate disappearance is very rapid at physiological pH. Activity can be measured as described in this unit by a number of indirect competitive inhibition assays based on the principle that the superoxide anion radical will reduce an inhibitory substrate [such as nitroblue tetrazolium (NBT) or cytochrome c] and SOD activity will reduce the rate of reduction in a competitive fashion. The SOD-mediated inhibition of the indicator substrate reduction can then be quantitated and plotted as a function of the quantity of protein added to the reaction to construct an inhibition curve.
Details
- Title: Subtitle
- Measurement of Mn SOD and Cu Zn SOD Activity in Mammalian Tissue Homogenates
- Creators
- Douglas R. Spitz - University of IowaLarry W. Oberley - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Current Protocols in Toxicology, Vol.8(1), pp.7.5.1-7.5.11
- DOI
- 10.1002/0471140856.tx0705s08
- ISSN
- 1934-9254
- eISSN
- 1934-9262
- Language
- English
- Date published
- 05/2001
- Academic Unit
- Pathology; Fraternal Order of Eagles Diabetes Research Center; Radiation Oncology
- Record Identifier
- 9984312963102771
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