Journal article
Measurement of contribution from intracellular cysteine to sulfate in phosphoadenosine phosphosulfate in rat ovarian granulosa cells
Archives of biochemistry and biophysics, Vol.312(2), pp.392-400
08/01/1994
DOI: 10.1006/abbi.1994.1324
PMID: 8037451
Abstract
Synthesis of the large dermatan sulfate (DS) proteoglycan by rat ovarian granulosa cells was studied using metabolic radiolabel precursors in culture media with varying concentrations of environmental sulfate (20-800 microM) and cysteine (130 and 650 microM). Experiments using [3H]glucosamine and [35S]sulfate showed that the average size of the DS chains and the rate of DS proteoglycan synthesis were independent of the sulfate and cysteine concentrations in the medium. Experiments with [35S]cysteine were then used to determine the contribution that metabolic conversion of cysteine sulfur to sulfate makes to the 3'-phosphoadenosine 5'-phosphosulfate (PAPS) pool which provides the substrate for sulfoester formation in DS synthesis. When 35S in cysteine is metabolized into [35S]PAPS, the specific activity is reduced from that of the [35S]cysteine pool, by dilution with other sulfur sources such as extracellular sulfate, and this dilution factor directly reflects the contribution of cysteine to the PAPS pool. The decreases of 35S specific activity were measured under various sulfate-depleted and cysteine-supplemented conditions by comparing the specific activity of [35S]sulfate ester in the DS chains with that of [35S]cysteine residues in the core protein of the DS proteoglycan. The contribution of sulfur in cysteine to the intracellular PAPS pool was 0.03% in culture medium with normal sulfate (800 microM). Depleted environmental sulfate (20 microM) and increased cysteine supply (650 microM) only increased the sulfur contribution from cysteine to PAPS up to 0.74 and 1.5%, respectively, even though the DS chains were greatly undersulfated (55 and 82% of the control value). Thus, the source of sulfur in the intracellular pool of PAPS was mainly derived from environmental sulfate, and the contribution from cysteine was minimal in these cells.
Details
- Title: Subtitle
- Measurement of contribution from intracellular cysteine to sulfate in phosphoadenosine phosphosulfate in rat ovarian granulosa cells
- Creators
- Y Imai - National Institutes of HealthM Yanagishita - Nidr, Bone Res Branch, Bethesda, MD 20892, USAV C Hascall - Nidr, Bone Res Branch, Bethesda, MD 20892, USA
- Resource Type
- Journal article
- Publication Details
- Archives of biochemistry and biophysics, Vol.312(2), pp.392-400
- DOI
- 10.1006/abbi.1994.1324
- PMID
- 8037451
- ISSN
- 0003-9861
- eISSN
- 1096-0384
- Language
- English
- Date published
- 08/01/1994
- Academic Unit
- Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984359578202771
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