Measurement of the T Cell Response to Preerythrocytic Vaccination in Mice

Jenna J Guthmiller; Ryan A Zander; Noah S Butler

doi:10.1007/978-1-4939-2815-6_2

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Measurement of the T Cell Response to Preerythrocytic Vaccination in Mice

Journal article

Open access

Measurement of the T Cell Response to Preerythrocytic Vaccination in Mice

Jenna J Guthmiller, Ryan A Zander and Noah S Butler

Methods in molecular biology (Clifton, N.J.), Vol.1325, pp.19-37

2015

DOI: 10.1007/978-1-4939-2815-6_2

PMCID: PMC5089868

PMID: 26450376

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https://www.ncbi.nlm.nih.gov/pmc/articles/5089868View

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Abstract

Whole attenuated parasite vaccines designed to elicit immunity against the clinically silent preerythrocytic stage of Plasmodium infection represent the most efficacious experimental platforms currently in clinical trial. Studies in rodents and humans show that T cells mediate vaccine-induced protection. Thus, determining the quantitative and qualitative properties of these T cells remains a major research focus. Most rodent models of preerythrocytic anti-Plasmodium vaccination focus on circumsporozoite-specific CD8 T cell responses in BALB/c mice. However, CD4 T cells and non-circumsporozoite-specific CD8 T cells also significantly contribute to protection. Here we describe alternative approaches that enable detection and functional characterization of total CD8 and CD4 T cell responses induced by preerythrocytic vaccination in mice. These flow cytometry-based approaches rely on monitoring the modulation of expressed integrins and co-receptors on the surface of T cells in vaccinated mice. The approaches enable direct determination of the magnitude, kinetics, distribution, phenotype, and functional features of T cell responses induced by infection or whole-parasite vaccination using any mouse-parasite species combination.

Erythrocytes - immunology

Plasmodium - pathogenicity

Humans

Malaria - immunology

Molecular Biology - methods

CD4-Positive T-Lymphocytes - immunology

Malaria - prevention & control

Vaccines, Attenuated - immunology

Animals

Vaccination - methods

Malaria - parasitology

Malaria Vaccines - immunology

Malaria Vaccines - therapeutic use

Mice

CD8-Positive T-Lymphocytes - immunology

Plasmodium - immunology

Details

Title: Subtitle: Measurement of the T Cell Response to Preerythrocytic Vaccination in Mice
Creators: Jenna J Guthmiller - Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma, OK, 73104, USA
Ryan A Zander - Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma, OK, 73104, USA
Noah S Butler - Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Biomedical Sciences Building, Room 1035, 940 Stanton L. Young Blvd., Oklahoma, OK, 73104, USA. noah-butler@ouhsc.edu
Resource Type: Journal article
Publication Details: Methods in molecular biology (Clifton, N.J.), Vol.1325, pp.19-37
DOI: 10.1007/978-1-4939-2815-6_2
PMID: 26450376
PMCID: PMC5089868
NLM abbreviation: Methods Mol Biol
eISBN: 9781493928156; 1493928155
ISSN: 1940-6029
eISSN: 1940-6029
Publisher: United States
Grant note: 5K22AI099070 / NIAID NIH HHS K22 AI099070 / NIAID NIH HHS P20 RR016478 / NCRR NIH HHS P20 GM103447 / NIGMS NIH HHS 8P20GM103447 / NIGMS NIH HHS
Language: English
Date published: 2015
Academic Unit: Microbiology and Immunology
Record Identifier: 9984001139602771

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