Measuring hyperemic response to light flicker stimulus using continuous laser speckle flowgraphy in mice

Michelle R Tamplin; Kimberly A Broadhurst; Anthony H Vitale; Ryuya Hashimoto; Randy H Kardon; Isabella M Grumbach

doi:10.1016/j.exer.2022.108952

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Measuring hyperemic response to light flicker stimulus using continuous laser speckle flowgraphy in mice

Journal article

Peer reviewed

Measuring hyperemic response to light flicker stimulus using continuous laser speckle flowgraphy in mice

Michelle R Tamplin, Kimberly A Broadhurst, Anthony H Vitale, Ryuya Hashimoto, Randy H Kardon and Isabella M Grumbach

Experimental eye research, Vol.216, pp.108952-108952

03/2022

DOI: 10.1016/j.exer.2022.108952

PMCID: PMC9014798

PMID: 35051429

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https://www.ncbi.nlm.nih.gov/pmc/articles/9014798View

Open Access

Abstract

Alterations in neurovascular coupling have been associated with various ocular, cerebral, and systemic vascular disorders. In the eye, changes in vessel caliber by dynamic vessel analysis have been used to measure neurovascular coupling following a light flicker stimulus. Here, we present a new protocol for quantifying light-flicker induced hyperemia in the C57/Bl6J mouse retina using laser speckle flowgraphy (LSFG). Our protocol was adapted from protocols used in human subjects. By acquiring continuous time series data, we detected significant increase in blood flow. These responses are maintained with low variability over multiple imaging sessions, indicating these methods may be applied in serial studies of neurovascular coupling.

Laser speckle flowgraphy

Neurovascular coupling

Retinal blood flow

Details

Title: Subtitle: Measuring hyperemic response to light flicker stimulus using continuous laser speckle flowgraphy in mice
Creators: Michelle R Tamplin - Department of Radiation Oncology, University of Iowa, Iowa City, IA, USA
Kimberly A Broadhurst - Division of Cardiovascular Medicine, Abboud Cardiovascular Research Center, Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
Anthony H Vitale - Division of Cardiovascular Medicine, Abboud Cardiovascular Research Center, Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
Ryuya Hashimoto - Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, IA, USA
Randy H Kardon - Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, IA, USA
Isabella M Grumbach - Department of Radiation Oncology, University of Iowa, Iowa City, IA, USA
Resource Type: Journal article
Publication Details: Experimental eye research, Vol.216, pp.108952-108952
DOI: 10.1016/j.exer.2022.108952
PMID: 35051429
PMCID: PMC9014798
NLM abbreviation: Exp Eye Res
ISSN: 0014-4835
eISSN: 1096-0007
Publisher: Elsevier Ltd
Grant note: DOI: 10.13039/100000002, name: National Institutes of Health; DOI: 10.13039/100000738, name: US Department of Veterans Affairs; DOI: 10.13039/100000968, name: American Heart Association Inc
Language: English
Date published: 03/2022
Academic Unit: Iowa Neuroscience Institute; Cardiovascular Medicine; Radiation Oncology; Internal Medicine; Ophthalmology and Visual Sciences
Record Identifier: 9984211795002771

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