Journal article
Measuring the link between cardiac mechanical function and metabolism during hyperpolarized C-13-pyruvate magnetic resonance experiments
Magnetic resonance imaging, Vol.68, pp.9-17
05/01/2020
DOI: 10.1016/j.mri.2020.01.009
PMCID: PMC7131884
PMID: 31978518
Abstract
Purpose: The goal of this study was to develop a methodology to investigate the relationship between contractile function and hyperpolarized (HP) [1-C-13]pyruvate metabolism in a small animal model. To achieve sufficient signal from HP C-13 compounds, HP C-13 MRS/MRSI has required relatively large infusion volumes relative to the total blood volume in small animal models, which may affect cardiac function.
Methods: Eight female Sprague Dawley rats were imaged on a 4.7T scanner with a dual tuned H-1/C-13 volume coil. ECG and respiratory gated k-t spiral MRSI and an IDEAL based reconstruction to determine [1-C-13]pyruvate metabolism in the myocardium. This was coupled with H-1 cine MRI to determine ventricular volumes and mechanical function pre- and post-infusion of [1-C-13]pyruvate. For comparison to the [1-C-13]pyruvate experiments, three female Sprague Dawley rats were imaged with H-1 cine MRI to determine myocardial function preand post-saline infusion.
Results: We demonstrated significant changes in cardiac contractile function between pre- and post-infusion of [1-C-13]pyruvate. Specifically, there was an increase in end-diastolic volume (EDV), stroke volume (SV), and ejection fraction (EF). Additionally, the ventricular vascular coupling ratio (VVCR) showed an improvement after [1-C-13]pyruvate infusion, indicating increased systolic performance due to an increased arterial load. There was a moderate to strong relationship between the downstream metabolic conversion of pyruvate to bicarbonate and a strong relationship between the conversion of pyruvate to lactate and the cardiac mechanical function response.
Conclusion: The infusion of [1-C-13]pyruvate resulted in demonstrable increases in contractile function which was related to pyruvate conversion to bicarbonate and lactate. The combined effects of the infusion volume and inotropic effects of pyruvate metabolism likely explains the augmentation in myocardial mechanical function seen in these experiments. Given the relationship between pyruvate metabolism and contractile function observed in this study, this methodological approach may be utilized to better understand cardiac metabolic and functional remodeling in heart disease.
Details
- Title: Subtitle
- Measuring the link between cardiac mechanical function and metabolism during hyperpolarized C-13-pyruvate magnetic resonance experiments
- Creators
- Gregory P. Barton - University of Wisconsin–MadisonErin B. Macdonald - University of Wisconsin–MadisonKara N. Goss - University of Wisconsin–MadisonMarlowe W. Eldridge - University of Wisconsin–MadisonSean B. Fain - University of Wisconsin–Madison
- Resource Type
- Journal article
- Publication Details
- Magnetic resonance imaging, Vol.68, pp.9-17
- Publisher
- Elsevier
- DOI
- 10.1016/j.mri.2020.01.009
- PMID
- 31978518
- PMCID
- PMC7131884
- ISSN
- 0730-725X
- eISSN
- 1873-5894
- Number of pages
- 9
- Grant note
- T32AI007635 / National Institute Of Allergy And Infectious Diseases of the National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) UL1TR000427 / NIH/NCATS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Advancing Translational Sciences (NCATS) Office of the Vice Chancellor for Research and Graduate Education at the University of Wisconsin-Madison Wisconsin Alumni Research Foundation (Fain)
- Language
- English
- Date published
- 05/01/2020
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Electrical and Computer Engineering; Health and Human Physiology
- Record Identifier
- 9984274956902771
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