Journal article
Mechanical stimulation of neurites generates an inward current in putative aortic baroreceptor neurons in vitro
Brain research, Vol.757(1), pp.149-154
1997
DOI: 10.1016/S0006-8993(97)00153-4
PMID: 9200510
Abstract
We investigated the responses of putative aortic baroreceptor neurons to mechanical stimulation of their processes. Putative aortic baroreceptor neurons were identified by applying the carbocyanine dye DiI to the adventitia of the aortic arch of anesthetized rats. After at least 1 week, the nodose ganglia were removed and the neurons were cultured. Within 2–3 days, neurite outgrowth was evident on many neurons. The soma was voltage-clamped using whole cell patch clamp techniques while the neurites were deformed with pneumatic ejection of bath solution at 5–15 psi using a glass pipette (7–15
μm) positioned at least 50
μm from the neurite. Mechanical stimulation induced an inward current in 15 out of 17 putative aortic baroreceptor neurons. The magnitude of the current was related to the intensity of stimulation. The current was blocked by 20
μM gadolinium (
n=11), a reported blocker of mechanically sensitive ion channels, or by incubating the cells overnight in 10
μM phalloidin, which binds to actin filaments (
n=5). We conclude that mechanical deformation of neurites of putative baroreceptor neurons activates a mechanosensitive inward current in the soma and that the cytoskeletal actin filaments are involved in the generation of this current.
Details
- Title: Subtitle
- Mechanical stimulation of neurites generates an inward current in putative aortic baroreceptor neurons in vitro
- Creators
- J.Thomas Cunningham - Department of Physiology, Dalton Cardiovascular Research Center, University of Missouri, Columbia, MO, USARuth E Wachtel - Department of Anesthesia, College of Medicine, University of Iowa, Iowa City, IA 52242, USAFrançois M Abboud - Department of Internal Medicine, SE 308 GH, College of Medicine, University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Brain research, Vol.757(1), pp.149-154
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0006-8993(97)00153-4
- PMID
- 9200510
- ISSN
- 0006-8993
- eISSN
- 1872-6240
- Language
- English
- Date published
- 1997
- Academic Unit
- Molecular Physiology and Biophysics; Cardiovascular Medicine; Anesthesia; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
- Record Identifier
- 9984025682102771
Metrics
17 Record Views