Journal article
Mechanism for Selective Synaptic Wiring of Rod Photoreceptors into the Retinal Circuitry and Its Role in Vision
Neuron (Cambridge, Mass.), Vol.87(6), pp.1248-1260
09/23/2015
DOI: 10.1016/j.neuron.2015.09.002
PMCID: PMC4583715
PMID: 26402607
Abstract
In the retina, rod and cone photoreceptors form distinct connections with different classes of downstream bipolar cells. However, the molecular mechanisms responsible for their selective connectivity are unknown. Here we identify a cell-adhesion protein, ELFN1, to be essential for the formation of synapses between rods and rod ON-bipolar cells in the primary rod pathway. ELFN1 is expressed selectively in rods where it is targeted to the axonal terminals by the synaptic release machinery. At the synapse, ELFN1 binds in trans to mGluR6, the postsynaptic receptor on rod ON-bipolar cells. Elimination of ELFN1 in mice prevents the formation of synaptic contacts involving rods, but not cones, allowing a dissection of the contributions of primary and secondary rod pathways to retinal circuit function and vision. We conclude that ELFN1 is necessary for the selective wiring of rods into the primary rod pathway and is required for high sensitivity of vision.
Details
- Title: Subtitle
- Mechanism for Selective Synaptic Wiring of Rod Photoreceptors into the Retinal Circuitry and Its Role in Vision
- Creators
- Yan Cao - Department of Neuroscience, The Scripps Research Institute, 130 Scripps Way, Jupiter, FL 33458, USAIgnacio Sarria - Department of Neuroscience, The Scripps Research Institute, 130 Scripps Way, Jupiter, FL 33458, USAKatherine E Fehlhaber - Jules Stein Eye Institute, Department of Ophthalmology, University of California, Los Angeles, 100 Stein Plaza, Los Angeles, CA 90095, USANaomi Kamasawa - Electron Microscopy Core Facility, Max Planck Florida Institute, 1 Max Planck Way, Jupiter, FL 33458, USACesare Orlandi - Department of Neuroscience, The Scripps Research Institute, 130 Scripps Way, Jupiter, FL 33458, USAKiely N James - Department of Molecular and Cellular Neuroscience, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92121, USAJennifer L Hazen - Department of Molecular and Cellular Neuroscience, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92121, USAMatthew R Gardner - Department of Infectious Disease, The Scripps Research Institute, 130 Scripps Way, Jupiter, FL 33458, USAMichael Farzan - Department of Infectious Disease, The Scripps Research Institute, 130 Scripps Way, Jupiter, FL 33458, USAAmy Lee - Department of Molecular Physiology and Biophysics, University of Iowa, 51 Newton Road, Iowa City, IA 52242, USASheila Baker - Department of Biochemistry, University of Iowa, 51 Newton Road, Iowa City, IA 52242, USAKristin Baldwin - Department of Molecular and Cellular Neuroscience, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92121, USAAlapakkam P Sampath - Jules Stein Eye Institute, Department of Ophthalmology, University of California, Los Angeles, 100 Stein Plaza, Los Angeles, CA 90095, USAKirill A Martemyanov - Department of Neuroscience, The Scripps Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA. Electronic address: kirill@scripps.edu
- Resource Type
- Journal article
- Publication Details
- Neuron (Cambridge, Mass.), Vol.87(6), pp.1248-1260
- Publisher
- United States
- DOI
- 10.1016/j.neuron.2015.09.002
- PMID
- 26402607
- PMCID
- PMC4583715
- ISSN
- 0896-6273
- eISSN
- 1097-4199
- Grant note
- R01 EY020542 / NEI NIH HHS DC009433 / NIDCD NIH HHS K02 DA026405 / NIDA NIH HHS R55 DC009433 / NIDCD NIH HHS R01 EY017606 / NEI NIH HHS R01 DC012592 / NIDCD NIH HHS R01 EY018139 / NEI NIH HHS EY018139 / NEI NIH HHS P30 EY000331 / NEI NIH HHS T32 EY007026 / NEI NIH HHS EY017606 / NEI NIH HHS R01 DC009433 / NIDCD NIH HHS NS084190 / NINDS NIH HHS EY000331 / NEI NIH HHS R01 NS084190 / NINDS NIH HHS
- Language
- English
- Date published
- 09/23/2015
- Academic Unit
- Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Biochemistry and Molecular Biology; University College Courses; Ophthalmology and Visual Sciences
- Record Identifier
- 9984025258702771
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