Mechanism of action of "ruthenium red" compounds on Ca2+ ionophore from sarcoplasmic reticulum (Ca2+ + Mg2+)- adenosine triphosphatase and lipid bilayer

Adil E Shamoo; Terence R Thompson; Kevin P Campbell; Terrence L Scott; David A Goldstein

doi:10.1016/S0021-9258(19)40849-1

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Mechanism of action of "ruthenium red" compounds on Ca2+ ionophore from sarcoplasmic reticulum (Ca2+ + Mg2+)- adenosine triphosphatase and lipid bilayer

Journal article

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Mechanism of action of "ruthenium red" compounds on Ca2+ ionophore from sarcoplasmic reticulum (Ca2+ + Mg2+)- adenosine triphosphatase and lipid bilayer

Adil E Shamoo, Terence R Thompson, Kevin P Campbell, Terrence L Scott and David A Goldstein

The Journal of biological chemistry, Vol.250(20), pp.8289-8291

10/25/1975

DOI: 10.1016/S0021-9258(19)40849-1

PMID: 126243

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Abstract

Sarcoplasmic reticulum (Ca2+ + Mg2+)-ATPase was previously shown to have Ca2+-dependent and -selective ionophoric activity when tested in oxidized cholesterol lipid bilayer membranes (Shamoo, A. E., and MacLennan, D. H. (1974) Proc. Natl. Acad. Sci. U. S. A. 71, 3522). ruthenium red, a known inhibitor of (Ca2+ + Mg2+)-ATPase, is found to inhibit the Ca2+-ionophoric activity associated with (Ca2+ + Mg2+)-ATPase. Furthermore, ruthenium red alone acts as an anion-selective ionophore in lipid bilayers with the the following selectivity sequence for anions: l- greater than Cl-, Br- greater than F- greater than NO3-. The PCl-/PNa+ ratio was approximately 4/l. The presence of ruthenium red in excess of Ca2+ ionophore in lipid bilayer experiments converts the cation selectivity of the bilayer due to Ca2+ ionophore into anion selectivity.

Cholesterol

Rabbits

Calcium - pharmacology

Ionophores - metabolism

Adenosine Triphosphatases - metabolism

Membranes, Artificial

Electric Conductivity

Enzyme Activation - drug effects

Magnesium - pharmacology

Ruthenium - pharmacology

Sarcoplasmic Reticulum - enzymology

Anions

Animals

Ruthenium Red - pharmacology

Models, Biological

Details

Title: Subtitle: Mechanism of action of "ruthenium red" compounds on Ca2+ ionophore from sarcoplasmic reticulum (Ca2+ + Mg2+)- adenosine triphosphatase and lipid bilayer
Creators: Adil E Shamoo
Terence R Thompson
Kevin P Campbell
Terrence L Scott
David A Goldstein
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.250(20), pp.8289-8291
DOI: 10.1016/S0021-9258(19)40849-1
PMID: 126243
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: United States
Language: English
Date published: 10/25/1975
Academic Unit: Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
Record Identifier: 9984068262202771

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