Journal article
Mechanism of nucleotide incorporation opposite a thymine-thymine dimer by yeast DNA polymerase η
Proceedings of the National Academy of Sciences - PNAS, Vol.100(21), pp.12093-12098
10/14/2003
DOI: 10.1073/pnas.2134223100
PMCID: PMC218718
PMID: 14527996
Abstract
DNA polymerase η (Polη) has the unique ability to replicate through UV-light-induced cyclobutane pyrimidine dimers. Here we use pre-steady-state kinetic analyses to examine the mechanism of nucleotide incorporation opposite a
cis
-
syn
thymine-thymine (TT) dimer and an identical nondamaged sequence by yeast Polη. Polη displayed “burst” kinetics for nucleotide incorporation opposite both the damaged and nondamaged templates. Although a slight decrease occurred in the affinity (
K
d
) of nucleotide binding opposite the TT dimer relative to the nondamaged template, the rate (
k
pol
) of nucleotide incorporation was the same whether the template was damaged or nondamaged. These results strongly support a mechanism in which the nucleotide is directly inserted opposite the TT dimer by using its intrinsic base-pairing ability without any hindrance from the distorted geometry of the lesion.
Details
- Title: Subtitle
- Mechanism of nucleotide incorporation opposite a thymine-thymine dimer by yeast DNA polymerase η
- Creators
- M. Todd Washington - Sealy Center for Molecular Science, University of Texas Medical Branch, 6.104 Blocker Medical Research Building, 11th and Mechanic Streets, Galveston, TX 77555-1061Louise Prakash - Sealy Center for Molecular Science, University of Texas Medical Branch, 6.104 Blocker Medical Research Building, 11th and Mechanic Streets, Galveston, TX 77555-1061Satya Prakash - Sealy Center for Molecular Science, University of Texas Medical Branch, 6.104 Blocker Medical Research Building, 11th and Mechanic Streets, Galveston, TX 77555-1061
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.100(21), pp.12093-12098
- DOI
- 10.1073/pnas.2134223100
- PMID
- 14527996
- PMCID
- PMC218718
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- National Academy of Sciences
- Language
- English
- Date published
- 10/14/2003
- Academic Unit
- Radiation Oncology; Biochemistry and Molecular Biology
- Record Identifier
- 9984024413502771
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