Journal article
Mechanism of p53 downstream effectors p21 and Gadd45 in DNA damage surveillance
Science in China. Series C, Life sciences, Vol.42(4), pp.427-434
08/1999
DOI: 10.1007/BF02882063
PMID: 18763134
Abstract
Both p21(WAF1/CIP1) and Gadd45 were activated in a p53-dependent manner in MCF-7 cells after being exposed to ionizing radiation. In order to investigate their roles in DNA damage suweillance, p21as/MCF-7 cells stably transfected by p21 antisense expression plasmid pC-WAF1-AS and Gadd45as/MCF-7 stably transfected by Gadd45 antisense expression plasmid pCMVas45 were established. It was observed that G1 arrest induced by radiation was significantly reduced in Gadd45as/MCF-7 cells as well as in p21as/MCF7 cells. Repair of radiation damaged report gene greatly reduced in Gadd45as/MCF-7 and p21asVMCF-7 cells. Apoptosis significantly increased in p21as/MCF-7 after exposure to radiation. These results suggest that both p21 and Gadd45 support cellular survival by taking roles in G1 arrest and DNA repair, furthermore, p21 protects cells from death by inhibiting apoptosis after exposure to ionizing radiation.
Details
- Title: Subtitle
- Mechanism of p53 downstream effectors p21 and Gadd45 in DNA damage surveillance
- Creators
- Xiangbing Meng - Department of Biochemistry, Institute of Radiation Medicine, Beijing, ChinaYan Dong - Department of Biochemistry, Institute of Radiation Medicine, Beijing, ChinaZhixian Sun - Department of Biochemistry, Institute of Radiation Medicine, Beijing, China
- Resource Type
- Journal article
- Publication Details
- Science in China. Series C, Life sciences, Vol.42(4), pp.427-434
- Publisher
- Science in China Press
- DOI
- 10.1007/BF02882063
- PMID
- 18763134
- ISSN
- 1006-9305
- eISSN
- 1862-2798
- Language
- English
- Date published
- 08/1999
- Academic Unit
- Pathology
- Record Identifier
- 9984186505902771
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