Mechanism of recruiting Sec6/8 (exocyst) complex to the apical junctional complex during polarization of epithelial cells

Charles Yeaman; Kent K Grindstaff; W James Nelson

doi:10.1242/jcs.00893

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Mechanism of recruiting Sec6/8 (exocyst) complex to the apical junctional complex during polarization of epithelial cells

Journal article

Peer reviewed

Mechanism of recruiting Sec6/8 (exocyst) complex to the apical junctional complex during polarization of epithelial cells

Charles Yeaman, Kent K Grindstaff and W James Nelson

Journal of cell science, Vol.117(Pt 4), pp.559-570

02/01/2004

DOI: 10.1242/jcs.00893

PMCID: PMC3368615

PMID: 14709721

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Abstract

Sec6/8 (exocyst) complex regulates vesicle delivery and polarized membrane growth in a variety of cells, but mechanisms regulating Sec6/8 localization are unknown. In epithelial cells, Sec6/8 complex is recruited to cell-cell contacts with a mixture of junctional proteins, but then sorts out to the apex of the lateral membrane with components of tight junction and nectin complexes. Sec6/8 complex fractionates in a high molecular mass complex with tight junction proteins and a portion of E-cadherin, and co-immunoprecipitates with cell surface-labeled E-cadherin and nectin-2alpha. Recruitment of Sec6/8 complex to cell-cell contacts can be achieved in fibroblasts when E-cadherin and nectin-2alpha are co-expressed. These results support a model in which localized recruitment of Sec6/8 complex to the plasma membrane by specific cell-cell adhesion complexes defines a site for vesicle delivery and polarized membrane growth during development of epithelial cell polarity.

Cell Line

Epithelial Cells - physiology

Animals

Carrier Proteins - metabolism

Cell Adhesion Molecules - physiology

Intracellular Membranes - physiology

Cell Adhesion - physiology

Cell Adhesion Molecules - isolation & purification

Dogs

Cell Polarity - physiology

Kidney - cytology

Macromolecular Substances

Details

Title: Subtitle: Mechanism of recruiting Sec6/8 (exocyst) complex to the apical junctional complex during polarization of epithelial cells
Creators: Charles Yeaman - Department of Molecular and Cellular Physiology, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, Stanford, CA 94305-5345, USA. charles-yeaman@uiowa.edu
Kent K Grindstaff
W James Nelson
Resource Type: Journal article
Publication Details: Journal of cell science, Vol.117(Pt 4), pp.559-570
DOI: 10.1242/jcs.00893
PMID: 14709721
PMCID: PMC3368615
NLM abbreviation: J Cell Sci
ISSN: 0021-9533
eISSN: 1477-9137
Publisher: England
Grant note: R01 GM035527 / NIGMS NIH HHS GM35227 / NIGMS NIH HHS
Language: English
Date published: 02/01/2004
Academic Unit: Anatomy and Cell Biology; Internal Medicine
Record Identifier: 9984025340202771

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