Journal article
Mechanisms of cellular resistance to hydrogen peroxide, hyperoxia, and 4-hydroxy-2-nonenal toxicity: The significance of increased catalase activity in H2O2-resistant fibroblasts
Archives of biochemistry and biophysics, Vol.292(1), pp.221-227
01/1992
DOI: 10.1016/0003-9861(92)90071-4
PMID: 1727639
Abstract
An H2O2-resistant variant (OC14) of the HA1 Chinese hamster fibroblast cell line which demonstrates a 20-fold increase in catalase activity was utilized in the study of mechanisms responsible for cellular resistance to hydrogen peroxide, oxygen, and 4-hydroxy-2-nonenal toxicity. HA1 and OC14 cells were treated with 9 mm aminotriazole which resulted in a 60 to 80% reduction in catalase activity. Pretreatment with aminotriazole resulted in significant sensitization to the toxicity of 1-h exposures to exogenously applied H2O2, which was proportional to the reduction in catalase activity. Treatment with aminotriazole produced significant sensitization to the toxicity of 95% O2 after 45 h of O2 exposure but no sensitization to the toxicity of a 1-h exposure to 50 μm 4-hydroxy-2-nonenal. Inhibition of catalase activity by aminotriazole had no effect on the metabolism of 4-hydroxy-2-nonenal by either cell line tested. These results support the conclusion that in H2O2-resistant cells, catalase activity is a major determinant of cellular resistance to H2O2 toxicity, whereas catalase activity has a limited role in cellular resistance to an acute exposure to 95% O2 and is unrelated to cellular resistance to 4-hydroxy-2-nonenal.
Details
- Title: Subtitle
- Mechanisms of cellular resistance to hydrogen peroxide, hyperoxia, and 4-hydroxy-2-nonenal toxicity: The significance of increased catalase activity in H2O2-resistant fibroblasts
- Creators
- Douglas R. Spitz - University of VirginiaDonna T. Adams - University of VirginiaC.Michael Sherman - University of VirginiaRobert J. Roberts - University of Virginia
- Resource Type
- Journal article
- Publication Details
- Archives of biochemistry and biophysics, Vol.292(1), pp.221-227
- DOI
- 10.1016/0003-9861(92)90071-4
- PMID
- 1727639
- ISSN
- 0003-9861
- eISSN
- 1096-0384
- Language
- English
- Date published
- 01/1992
- Academic Unit
- Pathology; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984313094102771
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