Journal article
Mechanosensitive currents in putative aortic baroreceptor neurons in vitro
Journal of neurophysiology, Vol.73(5), pp.2094-2098
05/01/1995
DOI: 10.1152/jn.1995.73.5.2094
PMID: 7623100
Abstract
1. Whole cell patch-clamp experiments were conducted to determine whether rat aortic baroreceptor neurons contain mechano-sensitive conductances. 2. Putative aortic baroreceptor neurons in the nodose ganglia were identified by injecting DiI onto the adventitia of the aortic arch. Nodose ganglia neurons were dissociated after > or = 1 wk. A fluorescein-conjugated tetanus toxin fragment was used to confirm that the cells labeled with DiI in culture were neurons. 3. Hypoosmotic stretch significantly increased the conductance of DiI-labeled neurons (n = 19). The reversal potential of the response was -11 +/- 1 (SE) mV. 4. In experiments on unlabeled neurons, only 7 of 13 cells showed increases in conductance. BC3H1 cells, a mouse tumor cell line, showed no changes in conductance. 5. Gadolinium (20 microM), a putative blocker of mechanosensitive channels, prevented the increase in conductance produced by hypoosmolality in seven of seven labeled cells. Equimolar concentrations of lanthanum (n = 6) and omega-conotoxin GVIA (1 microM, n = 4), which block voltage-gated calcium channels, failed to significantly affect the inward current.
Details
- Title: Subtitle
- Mechanosensitive currents in putative aortic baroreceptor neurons in vitro
- Creators
- J. T Cunningham - Department of Internal Medicine, College of Medicine, University ofIowa, Iowa City 52242, USAR. E Wachtel - Department of Internal Medicine, College of Medicine, University ofIowa, Iowa City 52242, USAF. M Abboud - Department of Internal Medicine, College of Medicine, University ofIowa, Iowa City 52242, USA
- Resource Type
- Journal article
- Publication Details
- Journal of neurophysiology, Vol.73(5), pp.2094-2098
- DOI
- 10.1152/jn.1995.73.5.2094
- PMID
- 7623100
- ISSN
- 0022-3077
- eISSN
- 1522-1598
- Language
- English
- Date published
- 05/01/1995
- Academic Unit
- Molecular Physiology and Biophysics; Cardiovascular Medicine; Anesthesia; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
- Record Identifier
- 9984025312602771
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