Journal article
Mechanosensitive ion channels in putative aortic baroreceptor neurons
American journal of physiology. Heart and circulatory physiology, Vol.275(4), pp.H1497-H1501
10/01/1998
DOI: 10.1152/ajpheart.1998.275.4.H1497
PMID: 9746502
Abstract
Cell-attached patch-clamp experiments were performed on dissociated neurons from nodose ganglia of adult rats. Putative aortic baroreceptor neurons were identified by labeling nerve endings in the adventitia of the aortic arch with the carbocyanine dye DiI. Whereas previous experiments demonstrated the presence of mechanosensitive (MS) whole cell currents, these experiments studied single MS ion channels and examined the influence of culture conditions on their expression. Single MS channels were activated by applying negative pressure through the recording pipette. Channel openings became more frequent as the negative pressure was increased, with open probability increasing significantly above 30 mmHg. MS channels had a slope conductance of 114 pS and a reversal potential of ∼0 mV, consistent with a nonspecific cation conductance. Channels were not affected by antagonists of voltage-gated conductances but were blocked by 20 μM gadolinium, a known blocker of MS ion channels. When nodose neurons were cocultured with aortic endothelial cells, but not aortic smooth muscle cells, the percentage of patches exhibiting MS ion channels increased significantly, suggesting that aortic endothelial cells secrete a diffusible factor that increases channel expression.
Details
- Title: Subtitle
- Mechanosensitive ion channels in putative aortic baroreceptor neurons
- Creators
- Shane Kraske - Cardiovascular Center, College of Medicine, University of Iowa, Iowa City 52242; and, Department of Veterans Affairs Medical Center, Iowa City, Iowa 52246J. Thomas Cunningham - Departments of Internal Medicine and, Cardiovascular Center, College of Medicine, University of Iowa, Iowa City 52242; andGeorge Hajduczok - Departments of Internal Medicine and, Cardiovascular Center, College of Medicine, University of Iowa, Iowa City 52242; andMark W Chapleau - Departments of Internal Medicine and, Cardiovascular Center, College of Medicine, University of Iowa, Iowa City 52242; and, Department of Veterans Affairs Medical Center, Iowa City, Iowa 52246Francois M Abboud - Departments of Internal Medicine and, Cardiovascular Center, College of Medicine, University of Iowa, Iowa City 52242; andRuth E Wachtel - Anesthesia and the, Cardiovascular Center, College of Medicine, University of Iowa, Iowa City 52242; and, Department of Veterans Affairs Medical Center, Iowa City, Iowa 52246
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Heart and circulatory physiology, Vol.275(4), pp.H1497-H1501
- DOI
- 10.1152/ajpheart.1998.275.4.H1497
- PMID
- 9746502
- ISSN
- 0363-6135
- eISSN
- 1522-1539
- Language
- English
- Date published
- 10/01/1998
- Academic Unit
- Molecular Physiology and Biophysics; Cardiovascular Medicine; Anesthesia; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
- Record Identifier
- 9984025438802771
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