Media from macrophages co-incubated with Enterococcus faecalis induces epithelial cell monolayer reassembly and altered cell morphology

Natalia Belogortseva; Monika Krezalek; Kristina Guyton; Christine Labno; Valeriy Poroyko; Olga Zaborina; John C Alverdy

doi:10.1371/journal.pone.0182825

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Media from macrophages co-incubated with Enterococcus faecalis induces epithelial cell monolayer reassembly and altered cell morphology

Journal article

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Peer reviewed

Media from macrophages co-incubated with Enterococcus faecalis induces epithelial cell monolayer reassembly and altered cell morphology

Natalia Belogortseva, Monika Krezalek, Kristina Guyton, Christine Labno, Valeriy Poroyko, Olga Zaborina and John C Alverdy

PloS one, Vol.12(8), pp.e0182825-e0182825

2017

DOI: 10.1371/journal.pone.0182825

PMCID: PMC5549984

PMID: 28793333

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Abstract

Signal exchange between intestinal epithelial cells, microbes and local immune cells is an important mechanism of intestinal homeostasis. Given that intestinal macrophages are in close proximity to both the intestinal epithelium and the microbiota, their pathologic interactions may result in epithelial damage. The present study demonstrates that co-incubation of murine macrophages with E. faecalis strains producing gelatinase (GelE) and serine protease (SprE) leads to resultant condition media (CM) capable of inducing reassembly of primary colonic epithelial cell monolayers. Following the conditioned media (CM) exposure, some epithelial cells are shed whereas adherent cells are observed to undergo dissolution of cell-cell junctions and morphologic transformation with actin cytoskeleton reorganization resulting in flattened and elongated shapes. These cells exhibit marked filamentous filopodia and lamellipodia formation. Cellular reorganization is not observed when epithelial monolayers are exposed to: CM from macrophages co-incubated with E. faecalis GelE/SprE-deficient mutants, CM from macrophages alone, or E. faecalis (GelE/SprE) alone. Flow cytometry analysis reveals increased expression of CD24 and CD44 in cells treated with macrophage/E. faecalis CM. This finding in combination with the appearance colony formation in matrigel demonstrate that the cells treated with macrophage/E. faecalis CM contain a higher proportion progenitor cells compared to untreated control. Taken together, these findings provide evidence for a triangulated molecular dialogue between E. faecalis, macrophages and colonic epithelial cells, which may have important implications for conditions in the gut that involve inflammation, injury or tumorigenesis.

Animals

CD24 Antigen - metabolism

Cell Line

Cell Shape - physiology

Culture Media

Enterococcus faecalis - metabolism

Epithelial Cells - cytology

Epithelial Cells - metabolism

Epithelial Cells - microbiology

Gelatinases - metabolism

Hyaluronan Receptors - metabolism

Intestinal Mucosa - cytology

Intestinal Mucosa - metabolism

Intestinal Mucosa - microbiology

Macrophages - cytology

Macrophages - metabolism

Macrophages - microbiology

Mice

Serine Endopeptidases - metabolism

Details

Title: Subtitle: Media from macrophages co-incubated with Enterococcus faecalis induces epithelial cell monolayer reassembly and altered cell morphology
Creators: Natalia Belogortseva - University of Chicago
Monika Krezalek - University of Chicago
Kristina Guyton - University of Chicago
Christine Labno - University of Chicago
Valeriy Poroyko - City Of Hope National Medical Center
Olga Zaborina - University of Chicago
John C Alverdy - University of Chicago
Resource Type: Journal article
Publication Details: PloS one, Vol.12(8), pp.e0182825-e0182825
DOI: 10.1371/journal.pone.0182825
PMID: 28793333
PMCID: PMC5549984
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Grant note: P30 DK042086 / NIDDK NIH HHS R01 GM062344 / NIGMS NIH HHS
Language: English
Date published: 2017
Academic Unit: Surgery
Record Identifier: 9984322941302771

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