Mediation of vascular relaxation in epineurial arterioles of the sciatic nerve: effect of diabetes in type 1 and type 2 diabetic rat models

Lawrence J Coppey; Jill S Gellett; Mark A Yorek

doi:10.1080/10623320303366

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Mediation of vascular relaxation in epineurial arterioles of the sciatic nerve: effect of diabetes in type 1 and type 2 diabetic rat models

Journal article

Peer reviewed

Mediation of vascular relaxation in epineurial arterioles of the sciatic nerve: effect of diabetes in type 1 and type 2 diabetic rat models

Lawrence J Coppey, Jill S Gellett and Mark A Yorek

Endothelium (New York, N.Y.), Vol.10(2), pp.89-94

2003

DOI: 10.1080/10623320303366

PMID: 12791516

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Abstract

Previously, the authors have reported that acetylcholine-induced vascular relaxation in epineurial arterioles of the sciatic nerve is mediated by nitric oxide (NO) and endothelium-derived hyperpolarizing factor (EDHF). Furthermore, they have demonstrated that acetylcholine-induced vasodilation in these vessels is impaired in streptozotocin-induced (type 1) and ZDF obese (type 2) diabetic rats. In the present study, the authors sought to determine the effect of diabetes on NO- and EDHF-mediated vasodilation in epineurial arterioles. In epineurial arterioles from nondiabetic Sprague-Dawley rats, NO and EDHF are equivalent in regard to their contribution to acetylcholine-induced vascular relaxation. In contrast, NO accounts for a greater portion of acetylcholine-induced vascular relaxation in normal glycemic ZDF lean rats. Following 4 weeks of hyperglycemia, the EDHF component of acetylcholine-induced vascular relaxation was totally inhibited in both streptozotocin-induced and ZDF obese diabetic rats. Vasodilation mediated by NO was still active in epineurial arterioles from both type 1 and type 2 diabetic rat models. These data suggest that diabetes causes an impairment in EDHF-mediated vascular relaxation and that interventions directed at improving EDHF production or bioactivity may improve vascular function in epineurial arterioles in diabetes.

Acetylcholine - pharmacology

Diabetes Mellitus, Type 1 - physiopathology

Endothelium, Vascular - physiopathology

Nitric Oxide Synthase - antagonists & inhibitors

Rats

Endothelium, Vascular - drug effects

Male

Sciatic Nerve - blood supply

Arterioles - drug effects

Rats, Sprague-Dawley

Indomethacin - pharmacology

Nitroarginine - pharmacology

Rats, Zucker

Animals

Diabetes Mellitus, Type 2 - physiopathology

Arterioles - physiopathology

Vasodilation - drug effects

Vasodilation - physiology

Biological Factors - pharmacology

Diabetes Mellitus, Experimental - physiopathology

Disease Models, Animal

Details

Title: Subtitle: Mediation of vascular relaxation in epineurial arterioles of the sciatic nerve: effect of diabetes in type 1 and type 2 diabetic rat models
Creators: Lawrence J Coppey - Veteran Affairs Medical Center, Diabetes Endocrinology Research Center, and Department of Internal Medicine, University of Iowa, Iowa City, IA 52246, USA
Jill S Gellett
Mark A Yorek
Resource Type: Journal article
Publication Details: Endothelium (New York, N.Y.), Vol.10(2), pp.89-94
DOI: 10.1080/10623320303366
PMID: 12791516
ISSN: 1062-3329
eISSN: 1029-2373
Grant note: DK58005 / NIDDK NIH HHS DK-25295 / NIDDK NIH HHS
Language: English
Date published: 2003
Academic Unit: Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
Record Identifier: 9984094594002771

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