Journal article
Meiotic Recombination Initiated by a Double-Strand Break in rad50Δ Yeast Cells Otherwise Unable to Initiate Meiotic Recombination
Genetics (Austin), Vol.143(2), pp.741-754
06/01/1996
DOI: 10.1093/genetics/143.2.741
PMCID: PMC1207333
PMID: 8725223
Abstract
Abstract Meiotic recombination in Saccharomyces cerevisiae is initiated by double-strand breaks (DSBs). We have developed a system to compare the properties of meiotic DSBs with those created by the site-specific HO endonuclease. HO endonuclease was expressed under the control of the meiotic-specific SPO13 promoter, creating a DSB at a single site on one of yeast's 16 chromosomes. In Rad+ strains the times of appearance of the HO-induced DSBs and of subsequent recombinants are coincident with those induced by normal meiotic DSBs. Physical monitoring of DNA showed that SPO13::HO induced gene conversions both in Rad+ and in rad50Δ cells that cannot initiate normal meiotic DSBs. We find that the RAD50 gene is important, but not essential, for recombination even after a DSB has been created in a meiotic cell. In rad50Δ cells, some DSBs are not repaired until a broken chromosome has been packaged into a spore and is subsequently germinated. This suggests that a broken chromosome does not signal an arrest of progression through meiosis. The recombination defect in rad50Δ diploids is not, however, meiotic specific, as mitotic rad50 diploids, experiencing an HO-induced DSB, exhibit similar departures from wild-type recombination.
Details
- Title: Subtitle
- Meiotic Recombination Initiated by a Double-Strand Break in rad50Δ Yeast Cells Otherwise Unable to Initiate Meiotic Recombination
- Creators
- A Malkova - Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02254-9110L Ross - Department of Microbiology, Tufts University, Boston, Massachusetts 02111D Dawson - Department of Microbiology, Tufts University, Boston, Massachusetts 02111Merl F Hoekstra - ICOS Corporation, Bothell, Washington 98201J E Haber - Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02254-9110
- Resource Type
- Journal article
- Publication Details
- Genetics (Austin), Vol.143(2), pp.741-754
- DOI
- 10.1093/genetics/143.2.741
- PMID
- 8725223
- PMCID
- PMC1207333
- ISSN
- 1943-2631
- eISSN
- 1943-2631
- Language
- English
- Date published
- 06/01/1996
- Academic Unit
- Biology
- Record Identifier
- 9984217526302771
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