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Melatonin improves deferoxamine antioxidant activity in protecting against lipid peroxidation caused by hydrogen peroxide in rat brain homogenates
Journal article   Peer reviewed

Melatonin improves deferoxamine antioxidant activity in protecting against lipid peroxidation caused by hydrogen peroxide in rat brain homogenates

S Ortega-Gutiérrez, J.J Garcı́a, E Martı́nez-Balları́n, R.J Reiter, S Millán-Plano, M Robinson and D Acuña-Castroviejo
Neuroscience letters, Vol.323(1), pp.55-59
2002
DOI: 10.1016/S0304-3940(01)02530-7
PMID: 11911989

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Abstract

Deferoxamine (DF) is an antioxidant molecule because of its ability to chelate iron. This study compared the ability of DF alone or in combination with melatonin, 5-methoxytryptophol or pinoline in preventing lipid peroxidation due to hydrogen peroxide (H 2O 2) in rat brain homogenates. Malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA) in the homogenates were measured as indices of lipid peroxidation. Incubation of homogenates with DF reduced, in a dose-dependent manner, MDA+4-HDA formation due to H 2O 2. When melatonin, 5-methoxytryptophol or pinoline were added to the incubation medium, the efficacy of DF in preventing lipid peroxidation was enhanced. These cooperative effects between DF, melatonin, and related pineal products may be important in protecting tissues from the oxidative stress due to iron overload.
Lipid peroxidation Melatonin Hydrogen peroxide Brain homogenates Deferoxamine

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