Melatonin reduces protein and lipid oxidative damage induced by homocysteine in rat brain homogenates

Santiago Ortega-Gutiérrez; Lorena Fuentes-Broto; Joaquín J García; Marta López-Vicente; Enrique Martínez-Ballarín; Francisco Javier Miana-Mena; Sergio Millán-Plano; Russel J Reiter

doi:10.1002/jcb.21327

Back

Melatonin reduces protein and lipid oxidative damage induced by homocysteine in rat brain homogenates

Journal article

Peer reviewed

Melatonin reduces protein and lipid oxidative damage induced by homocysteine in rat brain homogenates

Santiago Ortega-Gutiérrez, Lorena Fuentes-Broto, Joaquín J García, Marta López-Vicente, Enrique Martínez-Ballarín, Francisco Javier Miana-Mena, Sergio Millán-Plano and Russel J Reiter

Journal of cellular biochemistry, Vol.102(3), pp.729-735

10/15/2007

DOI: 10.1002/jcb.21327

PMID: 17427950

View Online

Abstract

Numerous data indicate that hyperhomocysteinemia is a risk factor for cardio- and cerebrovascular diseases. At least in part, homocysteine (HCY) impairs cerebrovascular function because it generates large numbers of free radicals. Since melatonin is a well-known antioxidant, which reduces oxidative stress and decreases HCY concentrations in plasma, the aim of this study was to investigate the effect of melatonin in preventing HCY-induced protein and lipid oxidation in rat brain homogenates. Brain homogenates were obtained from Sprague-Dawley rats and were incubated with or without HCY (0.01-5 mM) or melatonin (0.01-3 mM). Carbonyl content of proteins, and malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA) concentrations in the brain homogenates were used as an index of protein and lipid oxidation, respectively. Under the experimental conditions used, the addition of HCY (0.01-5 mM) to the homogenates enhanced carbonyl protein and MDA+4-HDA formation. Melatonin reduced, in a concentration-dependent manner, protein and lipid oxidation due to HCY in the brain homogenates. These data suggest that preserving proteins from oxidative insults is an additional mechanism by which melatonin may act as an agent in potentially decreasing cardiovascular and cerebrovascular diseases related to hyperhomocysteinemia.

Antioxidants - chemistry

Oxidative Stress

Antioxidants - metabolism

Humans

Oxidants - metabolism

Rats

Rats, Sprague-Dawley

Brain - metabolism

Dose-Response Relationship, Drug

Alkenes - chemistry

Animals

Time Factors

Models, Biological

Homocysteine - chemistry

Lipid Peroxidation

Melatonin - chemistry

Details

Title: Subtitle: Melatonin reduces protein and lipid oxidative damage induced by homocysteine in rat brain homogenates
Creators: Santiago Ortega-Gutiérrez - Department of Pharmacology and Physiology, University of Zaragoza, Zaragoza, Spain
Lorena Fuentes-Broto
Joaquín J García
Marta López-Vicente
Enrique Martínez-Ballarín
Francisco Javier Miana-Mena
Sergio Millán-Plano
Russel J Reiter
Resource Type: Journal article
Publication Details: Journal of cellular biochemistry, Vol.102(3), pp.729-735
DOI: 10.1002/jcb.21327
PMID: 17427950
NLM abbreviation: J Cell Biochem
ISSN: 0730-2312
eISSN: 1097-4644
Publisher: United States
Language: English
Date published: 10/15/2007
Academic Unit: Neurology; Radiology; Iowa Neuroscience Institute; Neurosurgery
Record Identifier: 9984013998702771

Metrics

8 Record Views

25 Times Cited - Web of Science