Journal article
Melting Temperature Mapping Method: A Novel Method for Rapid Identification of Unknown Pathogenic Microorganisms within Three Hours of Sample Collection
Scientific reports, Vol.5(1), pp.12543-12543
07/28/2015
DOI: 10.1038/srep12543
PMCID: PMC4517391
PMID: 26218169
Abstract
Acquiring the earliest possible identification of pathogenic microorganisms is critical for selecting the appropriate antimicrobial therapy in infected patients. We herein report the novel "melting temperature (Tm) mapping method" for rapidly identifying the dominant bacteria in a clinical sample from sterile sites. Employing only seven primer sets, more than 100 bacterial species can be identified. In particular, using the Difference Value, it is possible to identify samples suitable for Tm mapping identification. Moreover, this method can be used to rapidly diagnose the absence of bacteria in clinical samples. We tested the Tm mapping method using 200 whole blood samples obtained from patients with suspected sepsis, 85% (171/200) of which matched the culture results based on the detection level. A total of 130 samples were negative according to the Tm mapping method, 98% (128/130) of which were also negative based on the culture method. Meanwhile, 70 samples were positive according to the Tm mapping method, and of the 59 suitable for identification, 100% (59/59) exhibited a "match" or "broad match" with the culture or sequencing results. These findings were obtained within three hours of whole blood collection. The Tm mapping method is therefore useful for identifying infectious diseases requiring prompt treatment.
Details
- Title: Subtitle
- Melting Temperature Mapping Method: A Novel Method for Rapid Identification of Unknown Pathogenic Microorganisms within Three Hours of Sample Collection
- Creators
- Hideki Niimi - Clinical Laboratory Center, Toyama University Hospital, Toyama 930-0194, JapanTomohiro Ueno - Clinical Laboratory Center, Toyama University Hospital, Toyama 930-0194, JapanShirou Hayashi - Clinical Laboratory Center, Toyama University Hospital, Toyama 930-0194, JapanAkihito Abe - Kitami Information Technology Co., Ltd., Hokkaido 090-0813, JapanTakahiro Tsurue - Kitami Information Technology Co., Ltd., Hokkaido 090-0813, JapanMasashi Mori - Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, Ishikawa 921-8836, JapanHomare Tabata - Life Science Center, Hokkaido Mitsui Chemicals, Inc., Hokkaido 073-0138, JapanHiroshi Minami - Life Science Center, Hokkaido Mitsui Chemicals, Inc., Hokkaido 073-0138, JapanMichihiko Goto - Department of Internal Medicine, University of Iowa Carver College of Medicine, IA 52242, USA, and Iowa City Veterans Affairs Medical Center, IA 52246, USAMakoto Akiyama - Nagaresugi Geriatric Hospital, Toyama 939-8032, JapanYoshihiro Yamamoto - Department of Clinical Infectious Diseases, Toyama University Hospital, Toyama 930-0194, JapanShigeru Saito - Department of Obstetrics &Gynecology, Toyama University Hospital, Toyama 930-0194, JapanIsao Kitajima - Clinical Laboratory Center, Toyama University Hospital, Toyama 930-0194, Japan
- Resource Type
- Journal article
- Publication Details
- Scientific reports, Vol.5(1), pp.12543-12543
- DOI
- 10.1038/srep12543
- PMID
- 26218169
- PMCID
- PMC4517391
- NLM abbreviation
- Sci Rep
- ISSN
- 2045-2322
- eISSN
- 2045-2322
- Language
- English
- Date published
- 07/28/2015
- Academic Unit
- Infectious Diseases; Internal Medicine
- Record Identifier
- 9984094749202771
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