Journal article
Membrane lipid free radicals produced from L1210 murine leukemia cells by photofrin photosensitization: an electron paramagnetic resonance spin trapping study
Cancer research (Chicago, Ill.), Vol.53(16), pp.3670-3673
08/15/1993
PMID: 8393378
Abstract
We have detected membrane lipid-derived free radicals from neoplastic cells subjected to Photofrin photosensitization. The presence of the prooxidants iron or iron plus ascorbate in the L1210 cell system increased the intensity of the spin-trapped lipid radical electron paramagnetic resonance spectra and correspondingly decreased cell survival. In addition, raising the proportion of unsaturated lipids in the cell membranes by supplementation of the growth medium with docosahexaenoic acid increased lipid radical formation and decreased cell survival when the L1210 cells were subjected to Photofrin and light. These data educe the hypothesis that the extent of radical generation as well as the efficacy of photodynamic therapy can be increased when prooxidant conditions, which enhance free radical processes, are present in conjunction with photosensitizers that target membrane lipids.
Details
- Title: Subtitle
- Membrane lipid free radicals produced from L1210 murine leukemia cells by photofrin photosensitization: an electron paramagnetic resonance spin trapping study
- Creators
- G R Buettner - Department of Medicine, College of Medicine, University of Iowa, Iowa City 52242E E KelleyC P Burns
- Resource Type
- Journal article
- Publication Details
- Cancer research (Chicago, Ill.), Vol.53(16), pp.3670-3673
- Publisher
- United States
- PMID
- 8393378
- ISSN
- 0008-5472
- eISSN
- 1538-7445
- Grant note
- CA 31526 / NCI NIH HHS
- Language
- English
- Date published
- 08/15/1993
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984047885102771
Metrics
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