Mesoporous Silica Nanoparticle-Coated Microneedle Arrays for Intradermal Antigen Delivery

Jing Tu; Guangsheng Du; M Reza Nejadnik; Juha Mönkäre; Koen van der Maaden; Paul H H Bomans; Nico A J M Sommerdijk; Bram Slütter; Wim Jiskoot; Joke A Bouwstra; Alexander Kros

doi:10.1007/s11095-017-2177-4

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Mesoporous Silica Nanoparticle-Coated Microneedle Arrays for Intradermal Antigen Delivery

Journal article

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Mesoporous Silica Nanoparticle-Coated Microneedle Arrays for Intradermal Antigen Delivery

Jing Tu, Guangsheng Du, M Reza Nejadnik, Juha Mönkäre, Koen van der Maaden, Paul H H Bomans, Nico A J M Sommerdijk, Bram Slütter, Wim Jiskoot, Joke A Bouwstra, …

Pharmaceutical research, Vol.34(8), pp.1693-1706

08/01/2017

DOI: 10.1007/s11095-017-2177-4

PMCID: PMC5498618

PMID: 28536970

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Abstract

To develop a new intradermal antigen delivery system by coating microneedle arrays with lipid bilayer-coated, antigen-loaded mesoporous silica nanoparticles (LB-MSN-OVA). Synthesis of MSNs with 10-nm pores was performed and the nanoparticles were loaded with the model antigen ovalbumin (OVA), and coated with a lipid bilayer (LB-MSN-OVA). The uptake of LB-MSN-OVA by bone marrow-derived dendritic cells (BDMCs) was studied by flow cytometry. The designed LB-MSN-OVA were coated onto pH-sensitive pyridine-modified microneedle arrays and the delivery of LB-MSN-OVA into ex vivo human skin was studied. The synthesized MSNs demonstrated efficient loading of OVA with a maximum loading capacity of about 34% and the lipid bilayer enhanced the colloidal stability of the MSNs. Uptake of OVA loaded in LB-MSN-OVA by BMDCs was higher than that of free OVA, suggesting effective targeting of LB-MSN-OVA to antigen-presenting cells. Microneedles were readily coated with LB-MSN-OVA at pH 5.8, yielding 1.5 μg of encapsulated OVA per microneedle array. Finally, as a result of the pyridine modification, LB-MSN-OVA were effectively released from the microneedles upon piercing the skin. Microneedle arrays coated with LB-MSN-OVA were successfully developed and shown to be suitable for intradermal delivery of the encapsulated protein antigen.

Antigen-Presenting Cells - metabolism

Antigens - administration & dosage

Drug Carriers

Drug Liberation

Humans

Hydrogen-Ion Concentration

Injections, Intradermal

Lipid Bilayers

Macrophages - metabolism

Nanoparticles - chemistry

Needles

Ovalbumin - administration & dosage

Particle Size

Porosity

Silicon Dioxide - chemistry

Skin

Surface Properties

Details

Title: Subtitle: Mesoporous Silica Nanoparticle-Coated Microneedle Arrays for Intradermal Antigen Delivery
Creators: Jing Tu - Leiden University
Guangsheng Du - Leiden University
M Reza Nejadnik - Leiden University
Juha Mönkäre - Leiden University
Koen van der Maaden - Centre for Human Drug Research
Paul H H Bomans - Eindhoven University of Technology
Nico A J M Sommerdijk - Eindhoven University of Technology
Bram Slütter - Leiden University
Wim Jiskoot - Leiden University
Joke A Bouwstra - Centre for Human Drug Research
Alexander Kros - Leiden University
Resource Type: Journal article
Publication Details: Pharmaceutical research, Vol.34(8), pp.1693-1706
DOI: 10.1007/s11095-017-2177-4
PMID: 28536970
PMCID: PMC5498618
NLM abbreviation: Pharm Res
ISSN: 0724-8741
eISSN: 1573-904X
Grant note: DOI: 10.13039/501100004543, name: Chinese Scholarship Council
Language: English
Date published: 08/01/2017
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics
Record Identifier: 9984420837402771

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