Journal article
Messenger ribonucleic acid for the gonadal luteinizing hormone human chorionic gonadotropin receptor is not present in human endometrium
Fertility and sterility, Vol.71(2), pp.368-372
02/01/1999
DOI: 10.1016/S0015-0282(98)00453-1
Abstract
Objective: To determine whether messenger RNA for the gonadal LH/hCG receptor is present in human endometrium with the use of reverse-transcriptase polymerase chain reaction.
Design: In vitro experiment.
Setting: Academic medical center.
Patient(s): Premenopausal women who were not receiving hormonally active medications and who were undergoing hysterectomy for uterine leiomyomas, menorrhagia, pelvic pain, or uterine prolapse.
Intervention(s): Tissue from hysterectomy specimens was processed for RNA and treated with deoxyribonuclease where appropriate, and RNA was reverse-transcribed to complementary DNA.
Main Outcome Measure(s): An appropriately sized band after reverse-transcriptase polymerase chain reaction, followed by sequencing to confirm the results.
Result(s): A primer pair that spanned the extracellular domain was unable to amplify receptor complementary DNA from human endometrial tissue. For a primer pair that spanned transmembrane regions 2–6 of the receptor and was contained wholly in exon 11, a 552–base pair fragment was amplified successfully in 19 of 25 human endometrial samples.
Conclusion(s): The traditional gonadal LH/hCG receptor does not appear to be present in human endometrial tissue. The presence of a portion of the transmembrane part of the molecule suggests that human endometrium may express a truncated or variant form of the receptor.
Details
- Title: Subtitle
- Messenger ribonucleic acid for the gonadal luteinizing hormone human chorionic gonadotropin receptor is not present in human endometrium
- Creators
- Elizabeth A StewartMarine SahakianAlan RhoadesBradley J Voorhis - University of IowaRomana A Nowak
- Resource Type
- Journal article
- Publication Details
- Fertility and sterility, Vol.71(2), pp.368-372
- DOI
- 10.1016/S0015-0282(98)00453-1
- NLM abbreviation
- Fertil Steril
- ISSN
- 0015-0282
- Language
- English
- Date published
- 02/01/1999
- Academic Unit
- Obstetrics and Gynecology
- Record Identifier
- 9983557200502771
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