Journal article
Metabolic Control of Oocyte Apoptosis Mediated by 14-3-3ζ-Regulated Dephosphorylation of Caspase-2
Developmental cell, Vol.16(6), pp.856-866
2009
DOI: 10.1016/j.devcel.2009.04.005
PMCID: PMC2698816
PMID: 19531356
Abstract
Xenopus oocyte death is partly controlled by the apoptotic initiator caspase-2 (C2). We reported previously that oocyte nutrient depletion activates C2 upstream of mitochondrial cytochrome
c release. Conversely, nutrient-replete oocytes inhibit C2 via S135 phosphorylation catalyzed by calcium/calmodulin-dependent protein kinase II. We now show that C2 phosphorylated at S135 binds 14-3-3ζ, thus preventing C2 dephosphorylation. Moreover, we determined that S135 dephosphorylation is catalyzed by protein phosphatase-1 (PP1), which directly binds C2. Although C2 dephosphorylation is responsive to metabolism, neither PP1 activity nor binding is metabolically regulated. Rather, release of 14-3-3ζ from C2 is controlled by metabolism and allows for C2 dephosphorylation. Accordingly, a C2 mutant unable to bind 14-3-3ζ is highly susceptible to dephosphorylation. Although this mechanism was initially established in
Xenopus, we now demonstrate similar control of murine C2 by phosphorylation and 14-3-3 binding in mouse eggs. These findings provide an unexpected evolutionary link between 14-3-3 and metabolism in oocyte death.
Details
- Title: Subtitle
- Metabolic Control of Oocyte Apoptosis Mediated by 14-3-3ζ-Regulated Dephosphorylation of Caspase-2
- Creators
- Leta K. Nutt - Duke UniversityMarisa R. Buchakjian - Duke UniversityEugene Gan - Duke UniversityRashid Darbandi - Duke UniversitySook-Young Yoon - University of Massachusetts AmherstJudy Q. Wu - Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USAYuko J. Miyamoto - Elon UniversityJennifer A. Gibbon - Duke UniversityJosh L. Andersen - Duke UniversityChristopher D. Freel - Duke UniversityWanli Tang - Duke UniversityChangli He - University of Massachusetts AmherstManabu Kurokawa - Duke UniversityYongjun Wang - Albert Einstein College of MedicineSeth S. Margolis - Duke UniversityRafael A. Fissore - University of Massachusetts AmherstSally Kornbluth - Duke University
- Resource Type
- Journal article
- Publication Details
- Developmental cell, Vol.16(6), pp.856-866
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.devcel.2009.04.005
- PMID
- 19531356
- PMCID
- PMC2698816
- ISSN
- 1534-5807
- eISSN
- 1878-1551
- Language
- English
- Date published
- 2009
- Academic Unit
- Otolaryngology
- Record Identifier
- 9984312246402771
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