Metabolic flexibility of SUP05 under low DO growth conditions

T E Mattes; A E Ingalls; S Burke; R M Morris

doi:10.1111/1462-2920.15226

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Metabolic flexibility of SUP05 under low DO growth conditions

Journal article

Open access

Peer reviewed

Metabolic flexibility of SUP05 under low DO growth conditions

T E Mattes, A E Ingalls, S Burke and R M Morris

ENVIRONMENTAL MICROBIOLOGY, Vol.23(6), pp.2823-2833

2021

DOI: 10.1111/1462-2920.15226

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Abstract

Chemoautotrophic bacteria from the SUP05 clade often dominate anoxic waters within marine oxygen minimum zones (OMZs) where they use energy gained from the oxidation of reduced sulfur to fuel carbon fixation. Some of these SUP05 bacteria are facultative aerobes that can use either nitrate or oxygen as a terminal electron acceptor making them ideally suited to thrive at the boundaries of OMZs where they experience fluctuations in dissolved oxygen (DO). SUP05 metabolism in these regions, and therefore the biogeochemical function of SUP05, depends largely on their sensitivity to oxygen. We evaluated growth and quantified differences in gene expression inCa. T. autotrophicus strain EF1 from the SUP05 clade under high DO (22 mu M), anoxic, and low DO (3.8 mu M) concentrations. We show that strain EF1 cells respire oxygen and nitrate and that cells have higher growth rates, express more genes, and fix more carbon when oxygen becomes available for aerobic respiration. Evidence that facultatively aerobic SUP05 are more active and respire nitrate when oxygen becomes available at low concentrations suggests that they are an important source of nitrite across marine OMZ boundary layers.

ISRP Project 5 2020-2025

Details

Title: Subtitle: Metabolic flexibility of SUP05 under low DO growth conditions
Creators: T E Mattes - University of Iowa
A E Ingalls - University of Washington
S Burke - University of Washington
R M Morris - University of Washington
Resource Type: Journal article
Publication Details: ENVIRONMENTAL MICROBIOLOGY, Vol.23(6), pp.2823-2833
DOI: 10.1111/1462-2920.15226
ISSN: 1462-2920
Grant note: DOI: 10.13039/100007812, name: University of Washington; DOI: 10.13039/100000001, name: National Science Foundation; DOI: 10.13039/100000066, name: National Institute of Environmental Health Sciences, award: P42ES013661
Language: English
Date published: 2021
Academic Unit: Civil and Environmental Engineering; Iowa Superfund Research Program
Record Identifier: 9984239270402771

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