Journal article
Metabolically stabilized double-stranded mRNA polyplexes
Gene therapy, Vol.25(7), pp.473-484
10/2018
DOI: 10.1038/s41434-018-0038-3
PMCID: PMC6404535
PMID: 30154525
Abstract
The metabolic instability of mRNA currently limits its utility for gene therapy. Compared to plasmid DNA, mRNA is significantly more susceptible to digestion by RNase in the circulation following systemic dosing. To increase mRNA metabolic stability, we hybridized a complementary reverse mRNA with forward mRNA to generate double-stranded mRNA (dsmRNA). RNase A digestion of dsmRNA established a 3000-fold improved metabolic stability compared to single-stranded mRNA (ssmRNA). Formulation of a dsmRNA polyplex using a PEG-peptide further improved the stability by 3000-fold. Hydrodynamic dosing and quantitative bioluminescence imaging of luciferase expression in the liver of mice established the potent transfection efficiency of dsmRNA and dsmRNA polyplexes. However, hybridization of the reverse mRNA against the 5' and 3' UTR of forward mRNA resulted in UTR denaturation and a tenfold loss in expression. Repeat dosing of dsmRNA polyplexes produced an equivalent transient expression, suggesting the lack of an immune response in mice. Co-administration of excess uncapped dsmRNA with a dsmRNA polyplex failed to knock down expression, suggesting that dsmRNA is not a Dicer substrate. Maximal circulatory stability was achieved using a fully complementary dsmRNA polyplex. The results established dsmRNA as a novel metabolically stable and transfection-competent form of mRNA.
Details
- Title: Subtitle
- Metabolically stabilized double-stranded mRNA polyplexes
- Creators
- Jacob A Poliskey - Division of Medicinal and Natural Products Chemistry, College of Pharmacy, University of Iowa, Iowa City, IA, 52242, USASamuel T Crowley - Division of Medicinal and Natural Products Chemistry, College of Pharmacy, University of Iowa, Iowa City, IA, 52242, USARaghu Ramanathan - Division of Medicinal and Natural Products Chemistry, College of Pharmacy, University of Iowa, Iowa City, IA, 52242, USAChristopher W White - Division of Medicinal and Natural Products Chemistry, College of Pharmacy, University of Iowa, Iowa City, IA, 52242, USABasil Mathew - Division of Medicinal and Natural Products Chemistry, College of Pharmacy, University of Iowa, Iowa City, IA, 52242, USAKevin G Rice - Division of Medicinal and Natural Products Chemistry, College of Pharmacy, University of Iowa, Iowa City, IA, 52242, USA. kevin-rice@uiowa.edu
- Resource Type
- Journal article
- Publication Details
- Gene therapy, Vol.25(7), pp.473-484
- DOI
- 10.1038/s41434-018-0038-3
- PMID
- 30154525
- PMCID
- PMC6404535
- NLM abbreviation
- Gene Ther
- ISSN
- 0969-7128
- eISSN
- 1476-5462
- Publisher
- England
- Grant note
- GM117785 / NIGMS NIH HHS P30 DK054759 / NIDDK NIH HHS T32 GM00865 / NIGMS NIH HHS
- Language
- English
- Date published
- 10/2018
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984065320702771
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