Metabolism of glycitein (7,4'-dihydroxy-6-methoxy-isoflavone) by human gut microflora

Andrean L SIMONS; Mathieu RENOUF; Suzanne HENDRICH; Patricia A MURPHY

doi:10.1021/jf051546d

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Metabolism of glycitein (7,4'-dihydroxy-6-methoxy-isoflavone) by human gut microflora

Journal article

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Metabolism of glycitein (7,4'-dihydroxy-6-methoxy-isoflavone) by human gut microflora

Andrean L SIMONS, Mathieu RENOUF, Suzanne HENDRICH and Patricia A MURPHY

Journal of agricultural and food chemistry, Vol.53(22), pp.8519-8525

2005

DOI: 10.1021/jf051546d

PMID: 16248547

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Abstract

Gut microbial disappearance and metabolism of the soy isoflavone glycitein, 7,4'-dihydroxy-6-methoxyisoflavone, were investigated by incubating glycitein anaerobically with feces from 12 human subjects. The subjects' ages ranged from 24 to 53 years with a body mass index (BMI) of 20.9-25.8 kg/m(2) (mean BMI = 24.0 +/- 1.1 kg/m(2)). Glycitein disappearance followed an apparent first-order rate loss. Fecal glycitein disappearance rates for the subjects segregated into three different groups described as high (k = 0.67 +/- 0.14/h), moderate (k = 0.34 +/- 0.04/h), and low (k = 0.15 +/- 0.07/h) glycitein degraders (p < 0.0001). There was no dose effect on the disappearance rates for each subject from 10 to 250 microM glycitein (average k = 0.32 +/- 0.03/h, p > 0.05). Four putative glycitein metabolites, characterized by liquid chromatography-mass spectrometry (electrospray ionization using positive ionization mode), were dihydroglycitein, dihydro-6,7,4'-trihydroxyisoflavone, and 5'-O-methyl-O-desmethylangolensin. Two subjects produced a metabolite tentatively identified as 6-O-methyl-equol, and one subject produced daidzein as an additional metabolite of glycitein. These results show that glycitein is metabolized by human gut microorganisms and may follow metabolic pathways similar to other soy isoflavones.

Fundamental and applied biological sciences. Psychology

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Details

Title: Subtitle: Metabolism of glycitein (7,4'-dihydroxy-6-methoxy-isoflavone) by human gut microflora
Creators: Andrean L SIMONS - Department of Food Science and Human Nutrition, 2312 Food Sciences Building. Iowa State University, Ames, Iowa 50011, United States
Mathieu RENOUF - Department of Food Science and Human Nutrition, 2312 Food Sciences Building. Iowa State University, Ames, Iowa 50011, United States
Suzanne HENDRICH - Department of Food Science and Human Nutrition, 2312 Food Sciences Building. Iowa State University, Ames, Iowa 50011, United States
Patricia A MURPHY - Department of Food Science and Human Nutrition, 2312 Food Sciences Building. Iowa State University, Ames, Iowa 50011, United States
Resource Type: Journal article
Publication Details: Journal of agricultural and food chemistry, Vol.53(22), pp.8519-8525
DOI: 10.1021/jf051546d
PMID: 16248547
NLM abbreviation: J Agric Food Chem
ISSN: 0021-8561
eISSN: 1520-5118
Publisher: American Chemical Society; Washington, DC
Language: English
Date published: 2005
Academic Unit: Oral Pathology, Radiology and Medicine; Pathology; Pharmaceutical Sciences and Experimental Therapeutics; Radiation Oncology
Record Identifier: 9984046906802771

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