Journal article
Metabolomic characterization of renal ischemia and reperfusion in a swine model
Life sciences (1973), Vol.156, pp.57-67
07/01/2016
DOI: 10.1016/j.lfs.2016.05.025
PMID: 27208650
Abstract
Acute kidney injury (AKI) is a serious complication in hospitalized and transplanted patients, and is mainly caused by ischemia/reperfusion (I/R). However, the current diagnosis of AKI based on acute alterations in serum creatinine or urine output is late and unspecific. To identify new systemic biomarkers for AKI, we performed serum and urine metabolomic profile analyses during percutaneous unilateral renal I/R in a well-controlled swine model. For this, serial serum and urine samples obtained during the pre-ischemia, ischemia and reperfusion periods were analyzed by H-1 nuclear magnetic resonance at 600 MHz. Through the metabolic profiles over I/R, we identified eight serum metabolites that increased with ischemia and recovered to basal values after reperfusion, delineating the ischemic period. In addition, we identified 13 urinary metabolites that changed during the early reperfusion reflecting the ischemic kidney, being able to differentiate between pre-ischemia and post I/R periods. All selected metabolites are described in terms of disease pathophysiology (change of energetic pathway and oxidative stress), which suggest that these serum and urinary metabolites are candidate AKI biomarkers. Interestingly, the selected metabolites allowed us to identify, well described NF kappa B, leptin, INF-gamma and insulin pathways, and a new pathway (Huntingtin) that had not been previously implicated in renal I/R. Huntingtin showed different fragment patterns in ischemic versus non-ischemic kidneys. Therefore, the metabolomic profile found in renal I/R led to the identification of candidate disease biomarkers and a new pathway associated with renal injury. (C) 2016 Elsevier Inc. All rights reserved.
Details
- Title: Subtitle
- Metabolomic characterization of renal ischemia and reperfusion in a swine model
- Creators
- Pamella Araujo Malagrino - Universidade de São PauloGabriela Venturini - Universidade de São PauloPatricia Schneider Yogi - Universidade de São PauloRafael Dariolli - Universidade de São PauloKallyandra Padilha - Universidade de São PauloBianca Kiers - Universidade de São PauloTamiris Carneiro Gois - Universidade de São PauloJoaquim Mauricio Motta-Leal-Filho - Universidade de São PauloCelso Kiyochi Takimura - Universidade de São PauloAdriana Castello Costa Girardi - Universidade de São PauloFrancisco Cesar Carnevale - Universidade de São PauloRafael Canevarolo - Brazilian Biosciences National LaboratoryDenise Maria Avancini Costa MalheirosAna Carolina de Mattos Zeri - Brazilian Biosciences National LaboratoryJose Eduardo Krieger - Universidade de São PauloAlexandre Costa Pereira - Universidade de São Paulo
- Resource Type
- Journal article
- Publication Details
- Life sciences (1973), Vol.156, pp.57-67
- DOI
- 10.1016/j.lfs.2016.05.025
- PMID
- 27208650
- NLM abbreviation
- Life Sci
- ISSN
- 0024-3205
- eISSN
- 1879-0631
- Publisher
- Elsevier
- Number of pages
- 11
- Grant note
- 13/17368-0; 11/04344-0; 13/13526-0 / Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) 2011-04344 / Swedish Research Council Fleury/Finep 25000180672/2011-81 / Proadi Sus Samaritano 2011/04344-0; 2012/05447-0; 2012/12042-7; 2013/13526-0; 13/17368-0 / Fundacao de Amparo a Pesquisa (FAPESP); Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
- Language
- English
- Date published
- 07/01/2016
- Academic Unit
- Radiology
- Record Identifier
- 9985180934702771
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