Journal article
Metabonomic analysis of fatty acids in seminal plasma between healthy and asthenozoospermic men based on gas chromatography mass spectrometry
Andrologia, Vol.49(9), e12744
11/2017
DOI: 10.1111/and.12744
PMID: 28124472
Abstract
Summary
The mechanism of asthenozoospermia remains unclear. The knowledge of the metabolism of fatty acids in seminal plasma is important and meaningful for the pathological study of asthenozoospermia. We present an optimised assay of extraction and derivatisation followed by GC/MS to analyse metabolites, especially fatty acids, in seminal plasma from healthy and asthenozoospermic men. Eighty‐nine peaks including 17 kinds of fatty acids were analysed and identified in the chromatogram. The GC/MS data were analysed using t test, fold change and partial least squares discriminant analysis to explore the potential biomarkers of asthenozoospermia. Seven metabolites in asthenozoospermic group were found to be significantly different from those in the normal group (with p < .05, fold change >1.2 and variable importance for projection >1). Of which, high levels of oleic acid and palmitic acid in seminal plasma from asthenozoospermic men may indicate a membrane metabolism disorder in spermatozoa and the lack of valine in the asthenozoospermic group may contribute to poor sperm motility. The results may facilitate the understanding of the role of fatty acids and amino acids in asthenozoospermia and provide solid foundation for further pathological study of asthenozoospermia.
Details
- Title: Subtitle
- Metabonomic analysis of fatty acids in seminal plasma between healthy and asthenozoospermic men based on gas chromatography mass spectrometry
- Creators
- B Tang - Jinling HospitalX Shang - Jinling HospitalH Qi - Jinling HospitalJ Li - Jinling HospitalB Ma - Jinling HospitalG An - University of IowaQ Zhang - Jinling Hospital
- Resource Type
- Journal article
- Publication Details
- Andrologia, Vol.49(9), e12744
- DOI
- 10.1111/and.12744
- PMID
- 28124472
- ISSN
- 0303-4569
- eISSN
- 1439-0272
- Number of pages
- 13
- Grant note
- Specialized Research Fund for the Clinical Medicine of Jiangsu Province (SBL201330086) National Science and Technology Major Projects for “Major New Drugs Innovation and Development” (2013ZX09103001‐004) National Natural Science Foundation of China (81373478; 81370750)
- Language
- English
- Date published
- 11/2017
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics
- Record Identifier
- 9984065324202771
Metrics
27 Record Views