Metagenomic Sequencing of an Echovirus 30 Genome From Cerebrospinal Fluid of a Patient With Aseptic Meningitis and Orchitis

Anne Piantadosi; Shibani S Mukerji; Pooja Chitneni; Tracey A Cho; Lisa A Cosimi; Deborah T Hung; Marcia B Goldberg; Pardis C Sabeti; Daniel R Kuritzkes; Yonatan H Grad

doi:10.1093/ofid/ofx138

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Metagenomic Sequencing of an Echovirus 30 Genome From Cerebrospinal Fluid of a Patient With Aseptic Meningitis and Orchitis

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Metagenomic Sequencing of an Echovirus 30 Genome From Cerebrospinal Fluid of a Patient With Aseptic Meningitis and Orchitis

Anne Piantadosi, Shibani S Mukerji, Pooja Chitneni, Tracey A Cho, Lisa A Cosimi, Deborah T Hung, Marcia B Goldberg, Pardis C Sabeti, Daniel R Kuritzkes and Yonatan H Grad

Open forum infectious diseases, Vol.4(3), p.ofx138

06/01/2017

DOI: 10.1093/ofid/ofx138

PMCID: PMC5534216

PMID: 28761901

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Abstract

Enteroviruses cause a wide spectrum of clinical disease. In this study, we describe the case of a young man with orchitis and aseptic meningitis who was diagnosed with enterovirus infection. Using unbiased “metagenomic” massively parallel sequencing, we assembled a near-complete viral genome, the first use of this method for full-genome viral sequencing from cerebrospinal fluid. We found that the genome belonged to the subgroup echovirus 30, which is a common cause of aseptic meningitis but has not been previously reported to cause orchitis.

enterovirus

ID Case

metagenomics

orchitis

meningitis

Details

Title: Subtitle: Metagenomic Sequencing of an Echovirus 30 Genome From Cerebrospinal Fluid of a Patient With Aseptic Meningitis and Orchitis
Creators: Anne Piantadosi - Division of Infectious Disease, Department of Medicine
Shibani S Mukerji - Department of Neurology
Pooja Chitneni - Division of Infectious Disease, Department of Medicine
Tracey A Cho - Department of Neurology
Lisa A Cosimi - Harvard Medical School
Deborah T Hung - Department of Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital
Marcia B Goldberg - Division of Infectious Disease, Department of Medicine
Pardis C Sabeti - Broad Institute of MIT and Harvard
Daniel R Kuritzkes - Harvard Medical School
Yonatan H Grad - Harvard Medical School
Resource Type: Journal article
Publication Details: Open forum infectious diseases, Vol.4(3), p.ofx138
DOI: 10.1093/ofid/ofx138
PMID: 28761901
PMCID: PMC5534216
NLM abbreviation: Open Forum Infect Dis
ISSN: 2328-8957
eISSN: 2328-8957
Publisher: Oxford University Press; US
Language: English
Date published: 06/01/2017
Academic Unit: Neurology; Iowa Neuroscience Institute
Record Identifier: 9984020616302771

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