Journal article
Metagenomic analysis to identify novel infectious agents in systemic anaplastic large cell lymphoma
Infectious agents and cancer, Vol.16(1), pp.1-6
11/01/2021
DOI: 10.1186/s13027-021-00404-0
PMCID: PMC8591940
PMID: 34775986
Abstract
Abstract Systemic anaplastic large cell lymphoma (ALCL) is a rare CD30-expressing T-cell non-Hodgkin lymphoma. Risk of systemic ALCL is highly increased among immunosuppressed individuals. Because risk of cancers associated with viruses is increased with immunosuppression, we conducted a metagenomic analysis of systemic ALCL to determine whether a known or novel pathogen is associated with this malignancy. Total RNA was extracted and sequenced from formalin-fixed paraffin-embedded tumor specimens from 19 systemic ALCL cases (including one case from an immunosuppressed individual with human immunodeficiency virus infection), 3 Epstein-Barr virus positive diffuse large B-cell lymphomas (DLBCLs) occurring in solid organ transplant recipients (positive controls), and 3 breast cancers (negative controls). We used a pipeline based on the Genome Analysis Toolkit (GATK)-PathSeq algorithm to subtract out human RNA reads and map the remaining RNA reads to microbes. No microbial association with ALCL was identified, but we found Epstein-Barr virus in the DLBCL positive controls and determined the breast cancers to be negative. In conclusion, we did not find a pathogen associated with systemic ALCL, but because we analyzed only one ALCL tumor from an immunosuppressed person, we cannot exclude the possibility that a pathogen is associated with some cases that arise in the setting of immunosuppression.
Details
- Title: Subtitle
- Metagenomic analysis to identify novel infectious agents in systemic anaplastic large cell lymphoma
- Creators
- Parag Mahale - Infections and Immunoepidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer InstituteJason Nomburg - Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical SchoolJoo Y. Song - Department of Pathology, City of Hope National Medical CenterMia Steinberg - Cancer Genomics Research Laboratory, National Cancer InstituteGabriel Starrett - Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer InstituteJoseph Boland - Cancer Genomics Research Laboratory, National Cancer InstituteCharles F. Lynch - Department of Epidemiology, The University of Iowa College of Public HealthAmy Chadburn - Weill Cornell MedicinePaul G. Rubinstein - Stroger Hospital of Cook County, Ruth M. Rothstein Core Center, Rush University Medical CenterBrenda Y. Hernandez - University of Hawaii Cancer CenterDennis D. Weisenburger - Department of Pathology, City of Hope National Medical CenterSusan Bullman - Human Biology Division, Fred Hutchinson Cancer Research CenterEric A. Engels - Infections and Immunoepidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute
- Resource Type
- Journal article
- Publication Details
- Infectious agents and cancer, Vol.16(1), pp.1-6
- DOI
- 10.1186/s13027-021-00404-0
- PMID
- 34775986
- PMCID
- PMC8591940
- NLM abbreviation
- Infect Agent Cancer
- ISSN
- 1750-9378
- eISSN
- 1750-9378
- Publisher
- BMC
- Grant note
- DOI: 10.13039/100011541, name: Division of Cancer Epidemiology and Genetics, National Cancer Institute; DOI: 10.13039/100000054, name: National Cancer Institute
- Language
- English
- Date published
- 11/01/2021
- Academic Unit
- Epidemiology
- Record Identifier
- 9984214856002771
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