Metalloendopeptidase ADAM-like Decysin 1 (ADAMDEC1) in Colonic Subepithelial PDGFRα + Cells Is a New Marker for Inflammatory Bowel Disease

Se Eun Ha; Brian G Jorgensen; Lai Wei; Byungchang Jin; Min-Seob Kim; Sandra M Poudrier; Rajan Singh; Allison Bartlett; Hannah Zogg; Sei Kim; Gain Baek; Masaaki Kurahashi; Moon-Young Lee; Yong-Sung Kim; Suck-Chei Choi; Kent C Sasse; Samuel J S Rubin; Andres Gottfried-Blackmore; Laren Becker; Aida Habtezion; Kenton M Sanders; Seungil Ro

doi:10.3390/ijms23095007

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Metalloendopeptidase ADAM-like Decysin 1 (ADAMDEC1) in Colonic Subepithelial PDGFRα + Cells Is a New Marker for Inflammatory Bowel Disease

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Metalloendopeptidase ADAM-like Decysin 1 (ADAMDEC1) in Colonic Subepithelial PDGFRα + Cells Is a New Marker for Inflammatory Bowel Disease

Se Eun Ha, Brian G Jorgensen, Lai Wei, Byungchang Jin, Min-Seob Kim, Sandra M Poudrier, Rajan Singh, Allison Bartlett, Hannah Zogg, Sei Kim, …

International journal of molecular sciences, Vol.23(9), p.5007

04/30/2022

DOI: 10.3390/ijms23095007

PMCID: PMC9103908

PMID: 35563399

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Abstract

Metalloendopeptidase ADAM-Like Decysin 1 (ADAMDEC1) is an anti-inflammatory peptidase that is almost exclusively expressed in the gastrointestinal (GI) tract. We have recently found abundant and selective expression of Adamdec1 in colonic mucosal PDGFRα cells. However, the cellular origin for this gene expression is controversial as it is also known to be expressed in intestinal macrophages. We found that Adamdec1 mRNAs were selectively expressed in colonic mucosal subepithelial PDGFRα cells. ADAMDEC1 protein was mainly released from PDGFRα cells and accumulated in the mucosal layer lamina propria space near the epithelial basement membrane. PDGFRα cells significantly overexpressed Adamdec1 mRNAs and protein in DSS-induced colitis mice. Adamdec1 was predominantly expressed in CD45 PDGFRα cells in DSS-induced colitis mice, with only minimal expression in CD45 CD64 macrophages. Additionally, overexpression of both ADAMDEC1 mRNA and protein was consistently observed in PDGFRα cells, but not in CD64 macrophages found in human colonic mucosal tissue affected by Crohn's disease. In summary, PDGFRα cells selectively express ADAMDEC1, which is localized to the colon mucosa layer. ADAMDEC1 expression significantly increases in DSS-induced colitis affected mice and Crohn's disease affected human tissue, suggesting that this gene can serve as a diagnostic and/or therapeutic target for intestinal inflammation and Crohn's disease.

ADAM Proteins - genetics

ADAM Proteins - metabolism

Animals

Biomarkers

Colitis - chemically induced

Colitis - genetics

Colitis - metabolism

Colon - cytology

Colon - metabolism

Crohn Disease - metabolism

Inflammatory Bowel Diseases - diagnosis

Inflammatory Bowel Diseases - genetics

Inflammatory Bowel Diseases - metabolism

Intestinal Mucosa - metabolism

Mice

Receptor, Platelet-Derived Growth Factor alpha - metabolism

RNA, Messenger - genetics

RNA, Messenger - metabolism

Details

Title: Subtitle: Metalloendopeptidase ADAM-like Decysin 1 (ADAMDEC1) in Colonic Subepithelial PDGFRα + Cells Is a New Marker for Inflammatory Bowel Disease
Creators: Se Eun Ha - University of Nevada, Reno
Brian G Jorgensen - University of Nevada, Reno
Lai Wei - University of Nevada, Reno
Byungchang Jin - University of Nevada, Reno
Min-Seob Kim - Wonkwang University
Sandra M Poudrier - University of Nevada, Reno
Rajan Singh - University of Nevada, Reno
Allison Bartlett - University of Nevada, Reno
Hannah Zogg - University of Nevada, Reno
Sei Kim - University of Nevada, Reno
Gain Baek - University of Nevada, Reno
Masaaki Kurahashi - University of Iowa
Moon-Young Lee - Wonkwang University
Yong-Sung Kim - Wonkwang University
Suck-Chei Choi - Wonkwang University
Kent C Sasse - Sasse Surgical Associates, Reno, NV 89502, USA
Samuel J S Rubin - Stanford University
Andres Gottfried-Blackmore - Stanford University
Laren Becker - Stanford University
Aida Habtezion - Stanford University
Kenton M Sanders - University of Nevada, Reno
Seungil Ro - University of Nevada, Reno
Resource Type: Journal article
Publication Details: International journal of molecular sciences, Vol.23(9), p.5007
DOI: 10.3390/ijms23095007
PMID: 35563399
PMCID: PMC9103908
NLM abbreviation: Int J Mol Sci
ISSN: 1422-0067
eISSN: 1422-0067
Grant note: R01DK094886 / NIH HHS R01 DK094886 / NIDDK NIH HHS R01DK103055 / NIH HHS P01-DK41315 / NIH HHS R01 DK103055 / NIDDK NIH HHS P01 DK041315 / NIDDK NIH HHS R01 DK091336 / NIDDK NIH HHS
Language: English
Date published: 04/30/2022
Academic Unit: Internal Medicine
Record Identifier: 9984359841802771

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