Metastatic human hepatoblastoma cells exhibit enhanced tumorigenicity, invasiveness and a stem cell-like phenotype

Raoud Marayati; Janet R. Julson; Laura V. Bownes; Colin H. Quinn; Sara C. Hutchins; Adele P. Williams; Hooper R. Markert; Andee M. Beierle; Jerry E. Stewart; Anita B. Hjelmeland; Elizabeth Mroczek-Musulman; Elizabeth A. Beierle

doi:10.1016/j.jpedsurg.2022.01.063

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Metastatic human hepatoblastoma cells exhibit enhanced tumorigenicity, invasiveness and a stem cell-like phenotype

Journal article

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Metastatic human hepatoblastoma cells exhibit enhanced tumorigenicity, invasiveness and a stem cell-like phenotype

Raoud Marayati, Janet R. Julson, Laura V. Bownes, Colin H. Quinn, Sara C. Hutchins, Adele P. Williams, Hooper R. Markert, Andee M. Beierle, Jerry E. Stewart, Anita B. Hjelmeland, …

Journal of pediatric surgery, Vol.57(6), pp.1018-1025

06/01/2022

DOI: 10.1016/j.jpedsurg.2022.01.063

PMCID: PMC9119922

PMID: 35300860

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https://doi.org/10.1016/j.jpedsurg.2022.01.063View

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Abstract

Metastatic hepatoblastoma continues to pose a significant treatment challenge, primarily because the precise mechanisms involved in metastasis are not fully understood, making cell lines and preclinical models that depict the progression of disease and metastasis-related biology paramount. We aimed to generate and characterize a metastatic hepatoblastoma cell line to create a model for investigation of the molecular mechanisms associated with metastasis. Using a murine model of serial tail vein injections of the human hepatoblastoma HuH6 cell line, non-invasive bioluminescence imaging, and dissociation of metastatic pulmonary lesions, we successfully established and characterized the metastatic human hepatoblastoma cell line, HLM_3. The HLM_3 cells exhibited enhanced tumorigenicity and invasiveness, both in vitro and in vivo compared to the parent HuH6 cell line. Moreover, HLM_3 metastatic hepatoblastoma cells exhibited a stem cell-like phenotype and were more resistant to the standard chemotherapeutic cisplatin. This newly described metastatic hepatoblastoma cell line offers a novel tool to study mechanisms of tumor metastasis and evaluate new therapeutic strategies for metastatic hepatoblastoma.

Cisplatin

Hepatoblastoma

Metastases

Stem cell

Details

Title: Subtitle: Metastatic human hepatoblastoma cells exhibit enhanced tumorigenicity, invasiveness and a stem cell-like phenotype
Creators: Raoud Marayati - University of Alabama at Birmingham
Janet R. Julson - University of Alabama at Birmingham
Laura V. Bownes - University of Alabama at Birmingham
Colin H. Quinn - University of Alabama at Birmingham
Sara C. Hutchins - University of Alabama at Birmingham
Adele P. Williams - University of Alabama at Birmingham
Hooper R. Markert - University of Alabama at Birmingham
Andee M. Beierle - University of Alabama at Birmingham
Jerry E. Stewart - University of Alabama at Birmingham
Anita B. Hjelmeland - University of Alabama at Birmingham
Elizabeth Mroczek-Musulman - Department of Pathology, The Children's Hospital of Alabama, Birmingham, AL 35233, United States of America
Elizabeth A. Beierle - University of Alabama at Birmingham
Resource Type: Journal article
Publication Details: Journal of pediatric surgery, Vol.57(6), pp.1018-1025
DOI: 10.1016/j.jpedsurg.2022.01.063
PMID: 35300860
PMCID: PMC9119922
NLM abbreviation: J Pediatr Surg
ISSN: 0022-3468
eISSN: 1531-5037
Publisher: Elsevier Inc
Number of pages: 8
Language: English
Date published: 06/01/2022
Academic Unit: Stead Family Department of Pediatrics; Hematology/Oncology
Record Identifier: 9984701541502771

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