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Methamphetamine causes cardiovascular dysfunction via cystathionine gamma lyase and hydrogen sulfide depletion
Journal article   Open access   Peer reviewed

Methamphetamine causes cardiovascular dysfunction via cystathionine gamma lyase and hydrogen sulfide depletion

Gopi K. Kolluru, John D. Glawe, Sibile Pardue, Ahmad Kasabali, Shafiul Alam, Saranya Rajendran, Allison L. Cannon, Chowdhury S. Abdullah, James G. Traylor, Rodney E. Shackelford, …
Redox biology, Vol.57, pp.102480-102480
11/2022
DOI: 10.1016/j.redox.2022.102480
PMCID: PMC9513700
PMID: 36167027
url
https://doi.org/10.1016/j.redox.2022.102480View
Published (Version of record) Open Access

Abstract

Methamphetamine (METH) is an addictive illicit drug used worldwide that causes significant damage to blood vessels resulting in cardiovascular dysfunction. Recent studies highlight increased prevalence of cardiovascular disease (CVD) and associated complications including hypertension, vasospasm, left ventricular hypertrophy, and coronary artery disease in younger populations due to METH use. Here we report that METH administration in a mouse model of ‘binge and crash’ decreases cardiovascular function via cystathionine gamma lyase (CSE), hydrogen sulfide (H2S), nitric oxide (NO) (CSE/H2S/NO) dependent pathway. METH significantly reduced H2S and NO bioavailability in plasma and skeletal muscle tissues co-incident with a significant reduction in flow-mediated vasodilation (FMD) and blood flow velocity revealing endothelial dysfunction. METH administration also reduced cardiac ejection fraction (EF) and fractional shortening (FS) associated with increased tissue and perivascular fibrosis. Importantly, METH treatment selectively decreased CSE expression and sulfide bioavailability along with reduced eNOS phosphorylation and NO levels. Exogenous sulfide therapy or endothelial CSE transgenic overexpression corrected cardiovascular and associated pathological responses due to METH implicating a central molecular regulatory pathway for tissue pathology. These findings reveal that therapeutic intervention targeting CSE/H2S bioavailability may be useful in attenuating METH mediated cardiovascular disease. [Display omitted]
Cardiomyopathy Cystathionine gamma lyase Endothelial dysfunction Hydrogen sulfide Methamphetamine Nitric oxide

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