Method for the construction and use of carbon fiber multibarrel electrodes for deep brain recordings in the alert animal

Keiichiro Inagaki; Shane A. Heiney; Pablo M. Blazquez

doi:10.1016/j.jneumeth.2008.12.009

Back

Method for the construction and use of carbon fiber multibarrel electrodes for deep brain recordings in the alert animal

Journal article

Peer reviewed

Method for the construction and use of carbon fiber multibarrel electrodes for deep brain recordings in the alert animal

Keiichiro Inagaki, Shane A. Heiney and Pablo M. Blazquez

Journal of neuroscience methods, Vol.178(2), pp.255-262

04/15/2009

DOI: 10.1016/j.jneumeth.2008.12.009

PMCID: PMC2673794

PMID: 19135083

Files and links (1)

url

https://www.ncbi.nlm.nih.gov/pmc/articles/2673794View

Open Access

Abstract

Microiontophoresis of neuroactive substances during single unit recording in awake behaving animals can significantly advance our understanding of neural circuit function. Here, we present a detailed description of a method for constructing carbon fiber multibarrel electrodes suitable for delivering drugs while simultaneously recording single unit activity from deep structures, including brainstem nuclei and the cerebellum, in the awake behaving primate. We provide data that should aid in minimizing barrel resistance and the time required to fill long, thin multibarrel electrodes with solutions. We also show successful single unit recording from a variety of areas in the awake squirrel monkey central nervous system, including the vestibular nuclei, Interstitial Nucleus of Cajal, and the cerebellum. Our descriptions and data should be useful for investigators wishing to perform single unit recordings during microiontophoresis of neuroactive substances, particularly in deep structures of animals with chronically implanted recording chambers.

Electrophysiology

Extracellular recording

Iontophoresis

Multibarrel electrode

Pharmacology

Primate

Details

Title: Subtitle: Method for the construction and use of carbon fiber multibarrel electrodes for deep brain recordings in the alert animal
Creators: Keiichiro Inagaki - Department of Anatomy and Neurobiology, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110, United States
Shane A. Heiney - Washington University in St. Louis
Pablo M. Blazquez - Washington University in St. Louis
Resource Type: Journal article
Publication Details: Journal of neuroscience methods, Vol.178(2), pp.255-262
DOI: 10.1016/j.jneumeth.2008.12.009
PMID: 19135083
PMCID: PMC2673794
NLM abbreviation: J Neurosci Methods
ISSN: 0165-0270
eISSN: 1872-678X
Publisher: Elsevier B.V
Language: English
Date published: 04/15/2009
Academic Unit: Iowa Neuroscience Institute; Neuroscience and Pharmacology
Record Identifier: 9984622052402771

Metrics

9 Record Views

9 Times Cited - Web of Science