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Methods for the separation and identification of ribonucleotides on DEAE-cellulose
Journal article   Peer reviewed

Methods for the separation and identification of ribonucleotides on DEAE-cellulose

Youcef M. Rustum and Herbert S. Schwartz
Analytical biochemistry, Vol.53(2), pp.411-419
1973
DOI: 10.1016/0003-2697(73)90088-2
PMID: 4716378

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Abstract

Methods have been developed for the separation and identification of ribonucleotides. These methods include single gradient column chromatography with DEAE-cellulose type DE52 to separate purine and pyrimidine ribonucleotides, and PEI-cellulose to separate purine ribonucleotides. Sequential thin layer chromatography with PEI-cellulose may be adapted for the identification and confirmation of the separated components. Application of the methods is demonstrated with a rat liver extract.

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