Journal article
MiR-4319 Suppress the Malignancy of Triple-Negative Breast Cancer by Regulating Self-Renewal and Tumorigenesis of Stem Cells
Cellular physiology and biochemistry, Vol.48(2), pp.593-604
08/01/2018
DOI: 10.1159/000491888
PMID: 30021199
Abstract
Background/Aims: High levels of cancer stem cells (CSCs) in patients with triple-negative breast cancer (TNBC) correlate with risk of poor clinical outcome and possibly contribute to chemoresistance and metastasis in patients with highly malignant TNBC. Aberrant microRNA expression is associated with the dysfunction of self-renewal and proliferation in cancer stem cells, while there is little information about the TNBC-specific microRNAs in regulating CSC ability. Methods: Solexa deep sequencing was performed to detect the expression levels of TNBC or non-TNBC stem cells (CSCs) microRNAs. Mammosphere formation assay, qRT-PCR and the xenograft model in nude mice were performed. Bioinformatic analysis and microarray were used to select the target gene, and luciferase reporter assays were used to confirm the binding sites. Results: Solexa sequencing data exhibited differential expression of 193 microRNAs between TNBC and non-TNBC stem cells. The gene ontology analysis and pathways analyses showed that genes were involved in the maintenance of stemness. MiR-4319 could suppress the self-renewal and formation of tumorspheres in TNBC CSCs through E2F2, and also inhibited tumor initiation and metastasis in vivo. Moreover, increased E2F2 could reverse the effect of miR-4319 on the self-renewal in TNBC CSCs. Conclusions: MiR-4319 suppresses the malignancy of TNBC by regulating self-renewal and tumorigenesis of stem cells and might be a remarkable prognostic factor or therapeutic target for patients with TNBC. (c) 2018 The Author(s) Published by S. Karger AG, Basel
Details
- Title: Subtitle
- MiR-4319 Suppress the Malignancy of Triple-Negative Breast Cancer by Regulating Self-Renewal and Tumorigenesis of Stem Cells
- Creators
- Jiahui Chu - Nanjing Medical UniversityYongfei Li - Nanjing Medical UniversityXuemei Fan - Nanjing Maternity and Child Health Care HospitalJingjing Ma - Nanjing Medical UniversityJun Li - Nanjing Medical UniversityGuangping Lu - Nanjing Medical UniversityYanhong Zhang - Nanjing Medical UniversityYi Huang - Magee-Womens Research InstituteWei Li - Nanjing Medical UniversityXiang Huang - Nanjing Medical UniversityZiyi Fu - Nanjing Medical UniversityYongmei Yin - Nanjing Medical UniversityHongyan Yuan - Georgetown University Medical Center
- Resource Type
- Journal article
- Publication Details
- Cellular physiology and biochemistry, Vol.48(2), pp.593-604
- DOI
- 10.1159/000491888
- PMID
- 30021199
- NLM abbreviation
- Cell Physiol Biochem
- ISSN
- 1015-8987
- eISSN
- 1421-9778
- Publisher
- Karger
- Number of pages
- 12
- Grant note
- 81172503; 81302304; 81572603; 81570804 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) RC201153 / Key projects of Jiangsu Health Department 320.6750.17006 / Wu Jieping Medical Foundation BRA2017534 / Second Level of 333 Project of Jiangsu Province ZDRCA2016023 / Project of Jiangsu Provincial Medical Talent YKK17179 / General Project of Nanjing Health Bureau BRA2015470 / Third Level of 333 Project of Jiangsu Province QNRC2016095 / Project of Jiangsu Provincial Medical youth Talent
- Language
- English
- Date published
- 08/01/2018
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984383906602771
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