Journal article
MiR-93 enhances angiogenesis and metastasis by targeting LATS2
Cell cycle (Georgetown, Tex.), Vol.11(23), pp.4352-4365
12/01/2012
DOI: 10.4161/cc.22670
PMCID: PMC3552918
PMID: 23111389
Abstract
Here we report that miR-93, a miRNA in the miR-106B~25 cluster, a paralog of the miR-17–92 cluster, was significantly upregulated in human breast carcinoma tissues. We stably expressed miR-93 in the MT-1 human breast carcinoma cell line and found that tumors formed by the miR-93 cells contained more blood vessels than those formed by the control cells. Co-culture experiments indicated that the MT-1 cells displayed a high activity of adhesion with endothelial cells and could form larger and more tube-like structures with endothelial cells. Lung metastasis assays were performed in a mouse metastatic model, and it was found that expression of miR-93 promoted tumor cell metastasis to lung tissue. In cell culture, expression of miR-93 enhanced cell survival and invasion. We examined the potential target that mediated miR-93’s effects and found that the large tumor suppressor, homology 2 (LATS2) was a target of
miR-93
. Higher levels of LATS2 were associated with cell death in the tumor mass. Silencing LATS2 expression promoted cell survival, tube formation and invasion, while ectopic expression of LATS2 decreased cell survival and invasion. These findings demonstrated that miR-93 promoted tumor angiogenesis and metastasis by suppressing LATS2 expression. Our results suggest that the inhibition of
miR-93
function may be a feasible approach to repress tumor metastasis.
Details
- Title: Subtitle
- MiR-93 enhances angiogenesis and metastasis by targeting LATS2
- Creators
- Ling Fang - Sunnybrook Research Institute; Sunnybrook Health Sciences Centre; Toronto, ON CanadaWilliam W Du - Sunnybrook Research Institute; Sunnybrook Health Sciences Centre; Toronto, ON CanadaWeining Yang - Department of Biology; York University; Toronto, ON CanadaZina Jeyapalan Rutnam - Sunnybrook Research Institute; Sunnybrook Health Sciences Centre; Toronto, ON CanadaChun Peng - Department of Biology; York University; Toronto, ON CanadaHaoran Li - Sunnybrook Research Institute; Sunnybrook Health Sciences Centre; Toronto, ON CanadaYunxia Q O'Malley - Division of Surgical Oncology and Endocrine Surgery; University of Iowa Carver College of Medicine; Iowa City, IA USARyan W Askeland - Division of Surgical Oncology and Endocrine Surgery; University of Iowa Carver College of Medicine; Iowa City, IA USASonia Sugg - Division of Surgical Oncology and Endocrine Surgery; University of Iowa Carver College of Medicine; Iowa City, IA USAMingyao Liu - University Health Network; University of Toronto; Toronto, ON CanadaTanvi Mehta - Sunnybrook Research Institute; Sunnybrook Health Sciences Centre; Toronto, ON CanadaZhaoqun Deng - Sunnybrook Research Institute; Sunnybrook Health Sciences Centre; Toronto, ON CanadaBurton B Yang - Sunnybrook Research Institute; Sunnybrook Health Sciences Centre; Toronto, ON Canada
- Resource Type
- Journal article
- Publication Details
- Cell cycle (Georgetown, Tex.), Vol.11(23), pp.4352-4365
- Publisher
- Landes Bioscience
- DOI
- 10.4161/cc.22670
- PMID
- 23111389
- PMCID
- PMC3552918
- ISSN
- 1538-4101
- eISSN
- 1551-4005
- Language
- English
- Date published
- 12/01/2012
- Academic Unit
- Stead Family Department of Pediatrics; Surgery; Gastroenterology, Hepatology, Pancreatology, and Nutrition
- Record Identifier
- 9984051900002771
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