Journal article
MicroRNA-149 Negatively Regulates TLR-Triggered Inflammatory Response in Macrophages by Targeting MyD88: miR-149 Regulates Inflammatory Responses
Journal of cellular biochemistry, Vol.115(5), pp.919-927
05/2014
DOI: 10.1002/jcb.24734
Abstract
MicroRNAs (miRNAs) have been shown to be important regulators of TLR signaling pathway at the post-transcriptional level. In this study, the potential role of miR-149 was explored in murine alveolar macrophage RAW264.7 cells. Our results demonstrated a dynamic change of the expressions of miR-149 expression and MyD88 in macrophage RAW264.7 upon Mycobacterium bovis Bacillus Calmette-Guerlin (BCG) infection or lipopolysaccharide (LPS) stimulation. The presence of BCG or LPS dynamically reduced the miR-149 expression, along with a substantially striking increase of MyD88 expression in these cells. More importantly, overexpression of miR-149 in RAW264.7 cells was associated with a significant decrease of MyD88 protein expression, as well as a reduced production of inflammatory mediator NF-κB 1, TNF-α and IL-6 in response to BCG infection or LPS stimulation. Further studies using immunoblotting assay against anti-MyD88 antibody and microRNA targeting luciferase reporter assay revealed that miR-149 was able to directly target the 3′-UTR of MyD88 mRNA and post-transcriptionally regulated MyD88 protein expression. These data suggested that miR-149 might be a key player of immune modulator for TLR/MyD88 signaling pathway in macrophages, which may through a mechanism of negatively regulating MyD88-dependent Toll-like receptors signaling pathway. © 2013 Wiley Periodicals, Inc.
Details
- Title: Subtitle
- MicroRNA-149 Negatively Regulates TLR-Triggered Inflammatory Response in Macrophages by Targeting MyD88: miR-149 Regulates Inflammatory Responses
- Creators
- Guangxian XuZhaobo ZhangYiwen XingJun WeiZhaohui GeXiaoming LiuYing ZhangXuelan Huang
- Resource Type
- Journal article
- Publication Details
- Journal of cellular biochemistry, Vol.115(5), pp.919-927
- DOI
- 10.1002/jcb.24734
- ISSN
- 0730-2312
- eISSN
- 1097-4644
- Language
- English
- Date published
- 05/2014
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984288757502771
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