Journal article
MicroRNA-183 family expression in hair cell development and requirement of microRNAs for hair cell maintenance and survival
Developmental dynamics, Vol.240(4), pp.808-819
04/2011
DOI: 10.1002/dvdy.22591
PMCID: PMC3072272
PMID: 21360794
Abstract
MicroRNAs (miRNAs) post-transcriptionally repress complementary target gene expression and can contribute to cell differentiation. The coordinate expression of miRNA-183 family members (miR-183, miR-96, and miR-182) has been demonstrated in sensory cells of the mouse inner ear and other vertebrate sensory organs. To further examine hair cell miRNA expression in the mouse inner ear, we have analyzed miR-183 family expression in wild type animals and various mutants with defects in neurosensory development. miR-183 family member expression follows neurosensory cell specification, exhibits longitudinal (basal-apical) gradients in maturating cochlear hair cells, and is maintained in sensory neurons and most hair cells into adulthood. Depletion of hair cell miRNAs resulting from\nDicer1\nconditional knockout (CKO) in\nAtoh1-Cre\ntransgenic mice leads to more disparate basal-apical gene expression profiles and eventual hair cell loss. Results suggest that hair cell miRNAs subdue cochlear gradient gene expression and are required for hair cell maintenance and survival.
Details
- Title: Subtitle
- MicroRNA-183 family expression in hair cell development and requirement of microRNAs for hair cell maintenance and survival
- Creators
- Michael D Weston - Creighton University School of Dentistry, 2500 California Plaza, Omaha, NE 68178, USAMarsha L Pierce - Department of Biomedical Sciences, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178, USAHeather C Jensen-Smith - Department of Biomedical Sciences, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178, USABernd Fritzsch - Department of Biology, University of Iowa, 143 Biology Building, Iowa City, IA 52242, USASonia Rocha-Sanchez - Creighton University School of Dentistry, 2500 California Plaza, Omaha, NE 68178, USAKirk W Beisel - Department of Biomedical Sciences, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178, USAGarrett A Soukup - Department of Biomedical Sciences, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178, USA
- Resource Type
- Journal article
- Publication Details
- Developmental dynamics, Vol.240(4), pp.808-819
- DOI
- 10.1002/dvdy.22591
- PMID
- 21360794
- PMCID
- PMC3072272
- NLM abbreviation
- Dev Dyn
- ISSN
- 1058-8388
- eISSN
- 1097-0177
- Language
- English
- Date published
- 04/2011
- Academic Unit
- Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984070618002771
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